Anti-5-methylcytidine/m5C antibody (STJ11103906)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal 5-methylcytidine/m5C antibody for use in DB and ELISA in species independent samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | DB/ELISA |
| Host | Rabbit |
| Reactivity | Species Independent |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | DB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Specificity | m5C |
Additional Info
| Background | RNA methylation plays a significant regulatory role in various of physiological activities and it has gradually become a hotspot of epigenetics in the past decade. 2 NA-O-methyladenosine (Am) , 2 NA-O-methylguanosine (Gm) , 2 NA-O-methylcytidine (Cm) , 2 NA-O-methyluridine (Um) , N 6-methyladenosine (m6A) , N 1-methylguanosine (m1G) , 5-methylcytidine (m5C) , and 5-methyluridine (m5U) are representative 2 NA-O-methylation and base-methylation modified epigenetic marks of RNA. 5-Methylcytosine (5mC) , a modified nucleobase derived from cytosine, is present in both DNA and RNA molecules. 5mC in DNA is an essential epigenetic mark associated with gene silencing and genome stability during embryonic development. 5mC in ribonucleoside, called 5-methylcytidine (m5C) , is involved in structural stability, aminoacylation and codon recognition of tRNA. In rRNA, m5C affects translational fidelity and tRNA recognition. m5C-modified nucleobase also have been identified in mRNA and other non-coding RNA but the biological functions are still unclear. m5C is detected not only in tissues and cells but also in urine. It is reported that the levels of m5C change in urine or tissues from patients with various diseases such as bladder, breast and colon cancers. |
Information sourced from Uniprot.org