Beta Actin

Beta Actin – What’s so special about it? Out of all the thousands and thousands of known proteins that exist within the human genome, actin is the most abundant

Read More...

IHC Guaranteed Antibodies

Discover range of IHC Guaranteed Products

Read More..

Antibody Validation Project

Join our Antibody Validation Project and trial free samples

Read More..

Western Blot Membrane Stripping

 

Read More..

Brief History of Antibodies

 

 

From Vaccines to monoclonals

Over the past two centuries research and treatment regarding the knowledge and applications of antibodies has come a long way from its early discoveries and basic understanding...

Read More..

St John's Laboratory - Blog

Antibody validation and research tips

    Recent Posts

    How to get the most out of your ELISA

    How to get the most out of your ELISA ELISA (enzyme-linked immunosorbent assay) is a common test for detecting and quantifying proteins, particularly antibodies. The ability of ELISA to quantify proteins is the reason you might choose this technique over qualitative methods, such as western blotting. The (very) basic steps...

    Recent Posts

    Using Rf value to calculate molecular weight

    Using Rf value to calculate molecular weight In a previous post, we looked at how to calculate the Rf value of a protein from a western blot. The formula is: Rf = Migration distance of the protein Migration distance of the dye front So why do we need the Rf...

    Recent Posts

    How to calculate the Rf value of a protein from western blot

    How to calculate the Rf value of a protein from western blot Western blot is a common tool for identifying, quantifying and determining the size of specific proteins. Essentially, western blotting allows a mixture of proteins to be separated using gel electrophoresis. The proteins are then transferred from the gel...

    Recent Posts

    Tips for Successful Western Blotting

    Tips for Successful Western Blotting Western blotting is a powerful tool for studying proteins. As a popular technique, encountered by everyone from first-year students to experienced researchers, western blotting has a number of functions. In general, it is useful for identifying, semi-quantifying (it can show relative protein levels, but not...

    Recent Posts

    Tips For Optimal Flow Cytometry

    Tips For Optimal Flow Cytometry Flow cytometry is a widely utilised laser-based technique which lends itself to the analysis of various physical and chemical characteristics in a particular cell population. The method has grown to be a popular technique within the realm of antibody research through its proven use in...
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5