• Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100
  • Immunofluorescence analysis of human-kidney tissue. 1, ABCG2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Western blot analysis of NIH-3T3, 293 cells using ABCG2 Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000
  • Immunofluorescence analysis of human-kidney tissue. 1, ABCG2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of human-lung-cancer tissue. 1, ABCG2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of human-lung-cancer tissue. 1, ABCG2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of human-breast-cancer tissue. 1, ABCG2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of human-breast-cancer tissue. 1, ABCG2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-ABCG2 antibody (461-510 aa) (STJ97253)

SKU:
STJ97253

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IF/WB/IHC/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Broad substrate specificity ATP-binding cassette transporter ABCG2 (461-510 aa) is suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IF 1:50-200
WB 1:500-1:2000
IHC-P 1:100-1:300
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ABCG2
Gene ID: 9429
Uniprot ID: ABCG2_HUMAN
Immunogen Region: 461-510 aa
Specificity: ABCG2 Polyclonal Antibody detects endogenous levels of ABCG2 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human ABCG2 at the amino acid range 461-510
Post Translational Modifications N-glycosylated. Glycosylation-deficient ABCG2 is normally expressed and functional. Phosphorylated. Phosphorylation at Thr-362 by PIM1 is induced by drugs like mitoxantrone and is associated with cells increased drug resistance. It regulates the localization to the plasma membrane, the homooligomerization and therefore, the activity of the transporter.
Function Broad substrate specificity ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes a wide variety of physiological compounds, dietary toxins and xenobiotics from cells. Involved in porphyrin homeostasis, mediating the export of protoporphyrin IX (PPIX) from both mitochondria to cytosol and cytosol to extracellular space, it also functions in the cellular export of heme. Also mediates the efflux of sphingosine-1-P from cells. Acts as a urate exporter functioning in both renal and extrarenal urate excretion. In kidney, it also functions as a physiological exporter of the uremic toxin indoxyl sulfate. Also involved in the excretion of steroids like estrone 3-sulfate/E1S, 3beta-sulfooxy-androst-5-en-17-one/DHEAS, and other sulfate conjugates. Mediates the secretion of the riboflavin and biotin vitamins into milk. Extrudes pheophorbide a, a phototoxic porphyrin catabolite of chlorophyll, reducing its bioavailability. Plays an important role in the exclusion of xenobiotics from the brain (Probable). It confers to cells a resistance to multiple drugs and other xenobiotics including mitoxantrone, pheophorbide, camptothecin, methotrexate, azidothymidine, and the anthracyclines daunorubicin and doxorubicin, through the control of their efflux. In placenta, it limits the penetration of drugs from the maternal plasma into the fetus. May play a role in early stem cell self-renewal by blocking differentiation.
Protein Name Broad Substrate Specificity Atp-Binding Cassette Transporter Abcg2
Atp-Binding Cassette Sub-Family G Member 2
Breast Cancer Resistance Protein
Cdw338
Mitoxantrone Resistance-Associated Protein
Placenta-Specific Atp-Binding Cassette Transporter
Urate Exporter
Cd Antigen Cd338
Database Links Reactome: R-HSA-189451
Reactome: R-HSA-189483
Reactome: R-HSA-2161517
Reactome: R-HSA-917937
Reactome: R-HSA-9753281
Reactome: R-HSA-9793528
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Apical Cell Membrane
Mitochondrion Membrane
Enriched In Membrane Lipid Rafts
Alternative Antibody Names Anti-Broad Substrate Specificity Atp-Binding Cassette Transporter Abcg2 antibody
Anti-Atp-Binding Cassette Sub-Family G Member 2 antibody
Anti-Breast Cancer Resistance Protein antibody
Anti-Cdw338 antibody
Anti-Mitoxantrone Resistance-Associated Protein antibody
Anti-Placenta-Specific Atp-Binding Cassette Transporter antibody
Anti-Urate Exporter antibody
Anti-Cd Antigen Cd338 antibody
Anti-ABCG2 antibody
Anti-ABCP antibody
Anti-BCRP antibody
Anti-BCRP1 antibody
Anti-MXR antibody

Information sourced from Uniprot.org

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