Anti-ACADL antibody (31-210) (STJ22475)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ACADL (31-210) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ACADL |
| Gene ID | 33 |
| Uniprot ID | ACADL_HUMAN |
| Immunogen Region | 31-210 |
| Immunogen Sequence | GGEERLETPSAKKLTDIGIR RIFSPEHDIFRKSVRKFFQE EVIPHHSEWEKAGEVSREVW EKAGKQGLLGVNIAEHLGGI GGDLYSAAIVWEEQAYSNCS GPGFSIHSGIVMSYITNHGS EEQIKHFIPQMTAGKCIGAI AMTEPGAGSDLQGIKTNAKK DGSDWILNGSKVFISNGSLS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 31-210 of human ACADL (NP_001599.1). |
Additional Info
| Post Translational Modifications | Acetylation at Lys-318 and Lys-322 in proximity of the cofactor-binding sites strongly reduces catalytic activity. These sites are deacetylated by SIRT3. |
| Function | Long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Among the different mitochondrial acyl-CoA dehydrogenases, long-chain specific acyl-CoA dehydrogenase can act on saturated and unsaturated acyl-CoAs with 6 to 24 carbons with a preference for 8 to 18 carbons long primary chains. |
| Protein Name | Long-Chain Specific Acyl-Coa Dehydrogenase - MitochondrialLcad |
| Database Links | Reactome: R-HSA-77285Reactome: R-HSA-77288Reactome: R-HSA-77310 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Long-Chain Specific Acyl-Coa Dehydrogenase - Mitochondrial antibodyAnti-Lcad antibodyAnti-ACADL antibody |
Information sourced from Uniprot.org