| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-AMFR (384-643) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
AMFR |
| Gene ID: |
267 |
| Uniprot ID: |
AMFR_HUMAN |
| Immunogen Region: |
384-643 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 384-643 of human AMFR (NP_001135.3). |
| Immunogen Sequence: |
IADNNRVREEHQGENLDENL VPVAAAEGRPRLNQHNHFFH FDGSRIASWLPSFSVEVMHT TNILGITQASNSQLNAMAHQ IQEMFPQVPYHLVLQDLQLT RSVEITTDNILEGRIQVPFP TQRSDSIRPALNSPVERPSS DQEEGETSAQTERVPLDLSP RLEETLDFGEVEVEPSEVED FEARGSRFSKSADERQRMLV QRKDELLQQARKRFLNKSSE DDAASESFLPSEGASSDPV |
| Post Translational Modifications | Palmitoylation of the RING-type zing finger by ZDHHC6 promotes localization to the peripheral endoplasmic reticulum. |
| Function | E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins, such as CD3D, CYP3A4, CFTR, INSIG1, SOAT2/ACAT2 and APOB for proteasomal degradation. Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97-AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG1 complex at the ER membrane. In addition, interaction of AMFR with AUP1 facilitates interaction of AMFR with ubiquitin-conjugating enzyme UBE2G2 and ubiquitin ligase RNF139, leading to sterol-induced HMGCR ubiquitination. The ubiquitinated HMGCR is then released from the ER into the cytosol for subsequent destruction. In addition to ubiquitination on lysine residues, catalyzes ubiquitination on cysteine residues: together with INSIG1, mediates polyubiquitination of SOAT2/ACAT2 at 'Cys-277', leading to its degradation when the lipid levels are low. Catalyzes ubiquitination and subsequent degradation of INSIG1 when cells are depleted of sterols. Mediates polyubiquitination of INSIG2 at 'Cys-215' in some tissues, leading to its degradation. Also regulates ERAD through the ubiquitination of UBL4A a component of the BAG6/BAT3 complex. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor. In association with LMBR1L and UBAC2, negatively regulates the canonical Wnt signaling pathway in the lymphocytes by promoting the ubiquitin-mediated degradation of CTNNB1 and Wnt receptors FZD6 and LRP6. Regulates NF-kappa-B and MAPK signaling pathways by mediating 'Lys-27'-linked polyubiquitination of TAB3 and promoting subsequent TAK1/MAP3K7 activation. |
| Protein Name | E3 Ubiquitin-Protein Ligase AmfrAutocrine Motility Factor ReceptorAmf ReceptorRing Finger Protein 45Gp78 |
| Database Links | Reactome: R-HSA-532668Reactome: R-HSA-901032 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinPalmitoylation Promotes Localization To The Peripheral Endoplasmic Reticulum |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Amfr antibodyAnti-Autocrine Motility Factor Receptor antibodyAnti-Amf Receptor antibodyAnti-Ring Finger Protein 45 antibodyAnti-Gp78 antibodyAnti-AMFR antibodyAnti-RNF45 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
