Anti-ANKLE2 antibody (350-450) (STJ119707)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ119707
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General Information

Short DescriptionRabbit polyclonal ANKLE2 (350-450) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolANKLE2
Gene ID23141
Uniprot IDANKL2_HUMAN
Immunogen Region350-450
Immunogen SequenceCRYNVMHVAAKENQASICQL TLDVLENPDFMRLMYPDDDE AMLQKRIRYVVDLYLNTPDK MGYDTPLHFACKFGNADVVN VLSSHHLIVKNSRNKYDKTP E
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 350-450 of human ANKLE2 (NP_055929.1).

Additional Info

Function Involved in mitotic nuclear envelope reassembly by promoting dephosphorylation of BAF/BANF1 during mitotic exit. Coordinates the control of BAF/BANF1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of BAF/BANF1 by protein phosphatase 2A (PP2A), thereby facilitating nuclear envelope assembly. May regulate nuclear localization of VRK1 in non-dividing cells. It is unclear whether it acts as a real PP2A regulatory subunit or whether it is involved in recruitment of the PP2A complex. Involved in brain development.
Protein Name Ankyrin Repeat And Lem Domain-Containing Protein 2
Lem Domain-Containing Protein 4
Database Links Reactome: R-HSA-2995383
Reactome: R-HSA-9013404
Reactome: R-HSA-9013408
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Iii Membrane Protein
Alternative Antibody Names Anti-Ankyrin Repeat And Lem Domain-Containing Protein 2 antibody
Anti-Lem Domain-Containing Protein 4 antibody
Anti-ANKLE2 antibody
Anti-KIAA0692 antibody
Anti-LEM4 antibody

Information sourced from Uniprot.org

Citations

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