Anti-APLN antibody (28-77 aa) (STJ96528)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the C-terminal region of human APLN at the amino acid range 28-77
General Information
| Short Description | Rabbit polyclonal anti-Apelin (28-77 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC-P 1:100-300ELISA 1:20000IF 1:100-300 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | APLN |
| Gene ID | 8862 |
| Uniprot ID | APEL_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the C-terminal region of human APLN at the amino acid range 28-77 |
| Immunogen Region | 28-77 aa |
| Specificity | Apelin Polyclonal Antibody detects endogenous levels of Apelin protein. |
Additional Info
| Post Translational Modifications | Several active peptides may be produced by proteolytic processing of the peptide precursor. |
| Function | Peptide hormone that functions as endogenous ligand for the G-protein-coupled apelin receptor (APLNR/APJ), that plays a role in cadiovascular homeostasis. Functions as a balanced agonist activating both G(i) protein pathway and beta-arrestin pathway of APLNR. Downstream G proteins activation, apelin can inhibit cAMP production and activate key intracellular effectors such as ERKs. On the other hand, APLNR activation induces beta-arrestin recruitment to the membrane leading to desensitization and internalization of the receptor. Apelin blunts cardiac hypertrophic induction from APLNR on response to pathological stimuli, but also induces myocardial hypertrophy under normal conditions. Apelin-36 dissociates more hardly than (pyroglu)apelin-13 from APLNR. Involved in the regulation of cardiac precursor cell movements during gastrulation and heart morphogenesis. Has an inhibitory effect on cytokine production in response to T-cell receptor/CD3 cross-linking.the oral intake of apelin in the colostrum and the milk might therefore modulate immune responses in neonates. Plays a role in early coronary blood vessels formation. Mediates myocardial contractility in an ERK1/2-dependent manner. May also have a role in the central control of body fluid homeostasis by influencing vasopressin release and drinking behavior. (Microbial infection) Endogenous ligand for the apelin receptor (APLNR), an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APLNR. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. |
| Protein Name | ApelinApj Endogenous Ligand Cleaved Into - Apelin-36 - Apelin-31 - Apelin-28 - Apelin-13 |
| Database Links | Reactome: R-HSA-375276Reactome: R-HSA-418594 |
| Cellular Localisation | SecretedExtracellular SpaceAbundantly Secreted In The ColostrumLower Level In MilkDecreases Rapidly Within Several Days After Parturition In MilkBut Is Still Detectable Even In Commercial Milk |
| Alternative Antibody Names | Anti-Apelin antibodyAnti-Apj Endogenous Ligand Cleaved Into - Apelin-36 - Apelin-31 - Apelin-28 - Apelin-13 antibodyAnti-APLN antibodyAnti-APEL antibody |
Information sourced from Uniprot.org