• Western blot analysis of extracts of various cell lines, using ATP13A1 antibody (STJ115839) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-ATP13A1 antibody (880-960) (STJ115839)

SKU:
STJ115839

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATP13A1 (880-960) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP13A1
Gene ID: 57130
Uniprot ID: AT131_HUMAN
Immunogen Region: 880-960
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 880-960 of human ATP13A1 (NP_065143.2).
Immunogen Sequence: ALLANAPERVVERRRRPRDS PTLSNSGIRATSRTAKQRSG LPPSEEQPTSQRDRLSQVLR DLEDESTPIVKLGDASIAAP F
Function Endoplasmic reticulum translocase required to remove mitochondrial transmembrane proteins mistargeted to the endoplasmic reticulum. Acts as a dislocase that mediates the ATP-dependent extraction of mislocalized mitochondrial transmembrane proteins from the endoplasmic reticulum membrane. Specifically binds mitochondrial tail-anchored transmembrane proteins: has an atypically large substrate-binding pocket that recognizes and binds moderately hydrophobic transmembranes with short hydrophilic lumenal domains.
Protein Name Endoplasmic Reticulum Transmembrane Helix Translocase
Endoplasmic Reticulum P5a-Atpase
Database Links Reactome: R-HSA-936837
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Endoplasmic Reticulum Transmembrane Helix Translocase antibody
Anti-Endoplasmic Reticulum P5a-Atpase antibody
Anti-ATP13A1 antibody
Anti-ATP13A antibody
Anti-KIAA1825 antibody
Anti-CGI-152 antibody

Information sourced from Uniprot.org

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