Anti-Bak1 antibody (50-150) (STJ22759)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Bak (50-150) antibody for use in WB, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BAK1 |
| Gene ID | 578 |
| Uniprot ID | BAK_HUMAN |
| Immunogen Region | 50-150 |
| Immunogen Sequence | EGVAAPADPEMVTLPLQPSS TMGQVGRQLAIIGDDINRRY DSEFQTMLQHLQPTAENAYE YFTKIATSLFESGINWGRVV ALLGFGYRLALHVYQHGLTG F |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Bak (NP_001179.1). |
Additional Info
| Tissue Specificity | Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle. |
| Function | Plays a role in the mitochondrial apoptotic process. Upon arrival of cell death signals, promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM. This releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9 which in turn processes and activates the effector caspases. |
| Protein Name | Bcl-2 Homologous Antagonist/KillerApoptosis Regulator BakBcl-2-Like Protein 7Bcl2-L-7 |
| Database Links | Reactome: R-HSA-111452Reactome: R-HSA-111457Reactome: R-HSA-5620971 |
| Cellular Localisation | Mitochondrion Outer MembraneSingle-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Bcl-2 Homologous Antagonist/Killer antibodyAnti-Apoptosis Regulator Bak antibodyAnti-Bcl-2-Like Protein 7 antibodyAnti-Bcl2-L-7 antibodyAnti-BAK1 antibodyAnti-BAK antibodyAnti-BCL2L7 antibodyAnti-CDN1 antibody |
Information sourced from Uniprot.org