Anti-BAX antibody (90aa N-Term) (STJ140113)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
ImmunogenPurified recombinant peptide derived from within residues 90 aa to the N-terminus of human BAX produced in E. coli.
General Information
| Short Description | Goat polyclonal BCL2-associated X protein (90aa N-Term) antibody for use in WB, IHC-P, IHC-F and IF in human, mouse, rat, bovine, canine, chicken, avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep and simian samples. Dat |
| Applications | WB/IHC-P/IHC-F/IF |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 2 mg/mL |
| Purification | This antibody is epitope-affinity purified from goat antiserum. |
| Dilution Range | WB 1:500-1:5000IF 1:50-1:250IHC-F 1:250-1:1000IHC-P 1:250-1:1000Yu X He W Xie J et al. J Cardiovasc Electrophysiol. 2018 Dec. PMID: 30516302 |
| Formulation | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Storage Instruction | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Target Information
| Gene Symbol | BAX |
| Gene ID | 581 |
| Uniprot ID | BAX_HUMAN |
| Accession Number | ENSG00000087088 |
| Immunogen | Purified recombinant peptide derived from within residues 90 aa to the N-terminus of human BAX produced in E. coli. |
| Immunogen Region | 90aa N-Term |
| Immunogen Sequence | MDGSGEQPRGGGPTSSEQIM DGSGEQPRGGGPTSSEQIMK GSGEQPRGGGPTSSEQIMKT SGEQPRGGGPTSSEQIMKTG GEQPRGGGPTSSEQIMKTGA |
| Specificity | Detects a band of approximately 21 kDa by Western blot in the following cell lysate: hCat, At-T20, MDA-MB-231, H69 and HUH. |
Additional Info
| Tissue Specificity | Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. |
| Post Translational Modifications | Ubiquitinated in the absence of XRCC6/Ku70. Ubiquitination promotes protein degradation. Ubiquitinated on Lys-128 and Lys-190. 'Lys-63'-linked polyubiquitin chains on Lys-128 are removed by USP12. |
| Function | Plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. |
| Protein Name | Apoptosis Regulator BaxBcl-2-Like Protein 4Bcl2-L-4 |
| Database Links | Reactome: R-HSA-111457Reactome: R-HSA-114294Reactome: R-HSA-5620971Reactome: R-HSA-6803204Reactome: R-HSA-6804114Reactome: R-HSA-8878166Reactome: R-HSA-9603505 |
| Cellular Localisation | Isoform Alpha: Mitochondrion Outer MembraneSingle-Pass Membrane ProteinCytoplasmNucleusColocalizes With 14-3-3 Proteins In The CytoplasmUnder Stress ConditionsUndergoes A Conformation Change That Causes Release From Jnk-Phosphorylated 14-3-3 Proteins And Translocation To The Mitochondrion MembraneUpon Sendai Virus InfectionRecruited To The Mitochondrion Through Interaction With Irf3Isoform Beta: CytoplasmIsoform Gamma: CytoplasmIsoform Delta: Cytoplasm |
| Alternative Antibody Names | Anti-Apoptosis Regulator Bax antibodyAnti-Bcl-2-Like Protein 4 antibodyAnti-Bcl2-L-4 antibodyAnti-BAX antibodyAnti-BCL2L4 antibody |
Information sourced from Uniprot.org