| Host: | Rabbit |
| Applications: | CM/ELISA/IF/IHC/IP/WB |
| Reactivity: | Human/Monkey/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-BEST2 (388-404) is suitable for use in Confocal Microscopy, ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Biotin |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.75-1.2 µg/µl |
| Dilution Range: | WB: 1:500 DB: 1:10, 000 ELISA: 1:10, 000 IP: 1:200 IHC: 1:200 ICC: 1:200 IF: 1:200 CFM: 1:200 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | Best2 |
| Gene ID: | 212989 |
| Uniprot ID: | BEST2_MOUSE |
| Immunogen Region: | 388-404 |
| Immunogen: | Synthetic peptide taken within amino acid region 388-404 of mouse Bestrophin 2 protein, peptide was covalently modified. |
| Tissue Specificity | Expressed in mucin-secreting colonic goblet cells. |
| Function | Ligand-gated anion channel that allows the movement of anions across cell membranes when activated by calcium (Ca2+). Transports a large specter of anions, namely mediates the movement of chloride, L-glutamate and iodide. Calcium-binding triggers the dilation of the aperture, but calcium-dependent gating is only effective when the size of the passing anion is bigger than the closed aperture. Mediates the calcium-activated hydrogencarbonate movement and participates in colonic hydrogencarbonate secretion concomitant with mucin secretion. In non-pigmented epithelium (NPE), mediates the efflux of intracellular L-glutamate.binding of intracellular L-glutamate activates and open both the neck and the aperture of the channel, leading to L-glutamate exit promoting chloride influx movement from the extracellular side in trans. Also exhibits a directional permeability for intracellular glutamine, in a similar manner as for L-glutamate. |
| Protein Name | Bestrophin-2 Vitelliform Macular Dystrophy 2-Like Protein 1 |
| Database Links | Reactome: R-MMU-2672351 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein Basolateral Cell Membrane |
| Alternative Antibody Names | Anti-Bestrophin-2 antibody Anti-Vitelliform Macular Dystrophy 2-Like Protein 1 antibody Anti-Best2 antibody Anti-Vmd2l1 antibody |
Information sourced from Uniprot.org