Anti-β-actin antibody

[STJ96930] Download PDF Print Data Sheet
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Product name Anti-β-actin antibody
Short Description Mouse monoclonal to β-actin.
Description Beta actin is a protein encoded by the ACTB gene which is approximately 41,7 kDa. Beta actin is localised to the cytoplasm. It is involved in pathways such as blood-brain barrier and immune cell transmigration, regulation of actin cytoskeleton by Rho GTPases and development Slit-Robo signalling. This protein falls under the actin family. There are 3 main groups of actin isoforms in vertebrates namely, alpha, beta and gamma actins. Beta actin is a highly conserved proteins that is involved in various types of internal cell motility and are the major constituent of microfilaments. Beta actin is ubiquitously expressed in all eukaryotic cells. Mutations in the ACTB gene result in a form of juvenile-onset dystonia, which is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. Baraitser-Winter syndrome 1 is another disease that can occur due to mutations. This is rare developmental disorder that causes postnatal short stature and microcephaly, intellectual disability, seizures, and hearing loss. STJ96930 was developed from clone 60. The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This primary antibody binds endogenous beta actin.
Applications ICC, IHC, WB
Dilution range WB 1:5000
IHC 1:200
Specificity The antibody detects endogenous β-actin protein.
Protein Name Actin, cytoplasmic 1
Beta-actin
Actin, cytoplasmic 1, N-terminally processed
Immunogen Synthetic Peptide
Immunogen Region C-term
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Validated Application IHC WB
Host Mouse
Clonality Monoclonal
Reactivity Canine, Chicken, Hamster, Human, Insect, Monkey, Mouse, Rabbit, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Isotype IgG1
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 60
Gene Symbol ACTB
Database Links HGNC:132
OMIM:102630
Alternative Names β-actin
Actin, cytoplasmic 1 antibody
Beta-actin antibody
Actin, cytoplasmic 1, N-terminally processed antibody
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Post-translational Modifications ISGylated. Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization . Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding . The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners .
Cellular Localization Cytoplasm, cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Swiss-Prot Key P60709_HUMAN
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-18
I am interested in this antibody in 0.3mg size, 1mg/mL. Would it be possible to change the storage buffer to PBS and not include sodium azide?
Question by: Guest on 11 Apr 2018 12:40:00
-11
Hello, thank you for your query. If you would like to place a custom order, the product should be at least 1 mg. There will be no additional cost for changing the storage buffer. Please contact us at info@stjohnslabs.com for further information.
Answer by: Customer Support on 11 Apr 2018 12:43:00

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