![Anti-Bromodeoxyuridine antibody [IIB5] (STJ16100807)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/149524/346873/STJ16100807_1__72787.1681999029.png?c=1 "Anti-Bromodeoxyuridine antibody [IIB5] (STJ16100807)")
![Anti-Bromodeoxyuridine antibody [IIB5] (STJ16100807)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/149524/346874/STJ16100807_2__71419.1681999029.png?c=1 "Anti-Bromodeoxyuridine antibody [IIB5] (STJ16100807)")
![Anti-Bromodeoxyuridine antibody [IIB5] (STJ16100807)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/149524/346873/STJ16100807_1__72787.1681999029.png?c=1 "Anti-Bromodeoxyuridine antibody [IIB5] (STJ16100807)")
Anti-Bromodeoxyuridine antibody [IIB5] (STJ16100807)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenBrdU conjugated to Bovine Serum albumin
General Information
| Short Description | Mouse monoclonal anti-Bromodeoxyuridine for use in FC, IHC, IHC-F, IHC-P and ICC in Other samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC/IHC/IHC-F/IHC-P/ICC |
| Host | Mouse |
| Reactivity | Other |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | IIB5 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Dilution Range | FC 1:100-1:200IHCIHC-FIHC-P |
| Formulation | PBS with 0.09% sodium azide |
| Storage Instruction | Store at 2-8°C for up to 1-year, upon receipt. |
Target Information
| Immunogen | BrdU conjugated to Bovine Serum albumin |
Additional Info
| Background | The immunocytochemical detection of bromodeoxyuridine (BrdU) incorpoRated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorpoRated BrdU with specific anti-BrdU monoclonal antibodies is an accuRate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorpoRated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic prolifeRative information (such as the S-phase transit Rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. IIB5 reacts with bromodeoxyuridine (BrdU) also when incorporated into nuclear DNA.The antibody is known to cross-react with Iododeoxyuridine (IdU). Although we have no specific information concerning chlorodeoxyuridine (CldU) , it is to be expected that also this antigen is recognized by IIB5. |
Information sourced from Uniprot.org