• Western blot analysis of extracts of various cell lines, using CA4 antibody (STJ22858) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CA4 antibody (19-270) (STJ22858)

SKU:
STJ22858

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CA4 (19-270) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CA4
Gene ID: 762
Uniprot ID: CAH4_HUMAN
Immunogen Region: 19-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 19-270 of human CA4 (NP_000708.1).
Immunogen Sequence: AESHWCYEVQAESSNYPCLV PVKWGGNCQKDRQSPINIVT TKAKVDKKLGRFFFSGYDKK QTWTVQNNGHSVMMLLENKA SISGGGLPAPYQAKQLHLHW SDLPYKGSEHSLDGEHFAME MHIVHEKEKGTSRNVKEAQD PEDEIAVLAFLVEAGTQVNE GFQPLVEALSNIPKPEMSTT MAESSLLDLLPKEEKLRHYF RYLGSLTTPTCDEKVVWTVF REPIQLHREQILAFSQKLY
Tissue Specificity Expressed in the endothelium of the choriocapillaris in eyes (at protein level). Not expressed in the retinal epithelium at detectable levels.
Function Catalyzes the reversible hydration of carbon dioxide into bicarbonate and protons and thus is essential to maintaining intracellular and extracellular pH. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid.
Protein Name Carbonic Anhydrase 4
Carbonate Dehydratase Iv
Carbonic Anhydrase Iv
Ca-Iv
Database Links Reactome: R-HSA-1237044
Reactome: R-HSA-1247673
Reactome: R-HSA-1475029
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Alternative Antibody Names Anti-Carbonic Anhydrase 4 antibody
Anti-Carbonate Dehydratase Iv antibody
Anti-Carbonic Anhydrase Iv antibody
Anti-Ca-Iv antibody
Anti-CA4 antibody

Information sourced from Uniprot.org

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