Anti-Calsequestrin 1 antibody (100-200) [S5MR] (STJ11103065)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Calsequestrin 1 (100-200) antibody for use in WB, IF, ICC and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S5MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CASQ1 |
| Gene ID | 844 |
| Uniprot ID | CASQ1_HUMAN |
| Immunogen Region | 100-200 |
| Immunogen Sequence | EDKGVGFGLVDSEKDAAVAK KLGLTEVDSMYVFKGDEVIE YDGEFSADTIVEFLLDVLED PVELIEGERELQAFENIEDE IKLIGYFKSKDSEHYKAFED A |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human Calsequestrin 1 (NP_001222.3). |
Additional Info
| Tissue Specificity | Expressed in myoblasts (at protein level). |
| Post Translational Modifications | N-glycosylated. |
| Function | Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. Calcium ions are bound by clusters of acidic residues at the protein surface, often at the interface between subunits. Can bind around 80 Ca(2+) ions. Regulates the release of lumenal Ca(2+) via the calcium release channel RYR1.this plays an important role in triggering muscle contraction. Negatively regulates store-operated Ca(2+) entry (SOCE) activity. |
| Protein Name | Calsequestrin-1CalmitineCalsequestrin - Skeletal Muscle Isoform |
| Database Links | Reactome: R-HSA-2672351Reactome: R-HSA-5578775 |
| Cellular Localisation | Endoplasmic ReticulumSarcoplasmic ReticulumSarcoplasmic Reticulum LumenSarcoplasmic Reticulum MembranePeripheral Membrane ProteinLumenal SideMitochondrion MatrixThis Isoform Of Calsequestrin Occurs In The Sarcoplasmic Reticulum's Terminal Cisternae Luminal Spaces Of Fast Skeletal Muscle CellsPreferentially Forms Linear And Round Aggregates In The Endoplasmic Reticulum (Er) Of Resting CellsIn A Minority Of CellsHomogeneously Detected In The Er LumenColocalizes With Stim1 At Endoplasmic Reticulum In Response To A Depletion Of Intracellular Calcium |
| Alternative Antibody Names | Anti-Calsequestrin-1 antibodyAnti-Calmitine antibodyAnti-Calsequestrin - Skeletal Muscle Isoform antibodyAnti-CASQ1 antibodyAnti-CASQ antibody |
Information sourced from Uniprot.org