| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC |
| Reactivity: | Human/Mouse/Rat/Dog |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-Catenin alpha1 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000. WB: Approx 100kDa band observed in human colon and human skin lysates (calculated MW of 100kDa according to NP_001894.2). Recommended concentration: 1-3µg/ml. IHC: Paraffin embedded Hu |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | CTNNA1 |
| Gene ID: | 1495 |
| Uniprot ID: | CTNA1_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_001894.2 |
| Immunogen Sequence: | KREKQDETQTKIK |
| Post Translational Modifications | Sumoylated. Phosphorylation seems to contribute to the strength of cell-cell adhesion rather than to the basic capacity for cell-cell adhesion. |
| Function | Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. Involved in the regulation of WWTR1/TAZ, YAP1 and TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation. May play a crucial role in cell differentiation. |
| Protein Name | Catenin Alpha-1 Alpha E-Catenin Cadherin-Associated Protein Renal Carcinoma Antigen Ny-Ren-13 |
| Database Links | Reactome: R-HSA-418990 Reactome: R-HSA-5218920 Reactome: R-HSA-525793 Reactome: R-HSA-5626467 Reactome: R-HSA-9762292 Reactome: R-HSA-9764302 Reactome: R-HSA-9833576 |
| Cellular Localisation | Cytoplasm Cytoskeleton Cell Junction Adherens Junction Cell Membrane Peripheral Membrane Protein Cytoplasmic Side Nucleus Found At Cell-Cell Boundaries And Probably At Cell-Matrix Boundaries Isoform 3: Cell Membrane |
| Alternative Antibody Names | Anti-Catenin Alpha-1 antibody Anti-Alpha E-Catenin antibody Anti-Cadherin-Associated Protein antibody Anti-Renal Carcinoma Antigen Ny-Ren-13 antibody Anti-CTNNA1 antibody |
Information sourced from Uniprot.org
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