• Biotinylated STJ70728 (1µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-Caveolin 1, Biotinylated antibody (Internal) {Biotin} (STJ73171)

SKU:
STJ73171-100

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Host: Goat
Applications: Pep-ELISA/WB/ICC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Caveolin 1, Biotinylated (Internal) is suitable for use in ELISA, Western Blot and Immunocytochemistry research applications.
Clonality: Polyclonal
Conjugation: Biotin
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-3µg/ml
ELISA-antibody detection limit dilution 1:8000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: CAV1
Gene ID: 857
Uniprot ID: CAV1_HUMAN
Immunogen Region: Internal
Accession Number: NP_001744.2; NP_001166366.1
Specificity: This antibody is expected to recognize both reported isoforms (NP_001744.2; NP_001166366.1). Reported variants represent identical protein: NP_001166367.1, NP_001166368.1, NP_001166366.1. No cross-reactivity expected to Caveolin 2 and Caveolin 3
Immunogen Sequence: DELSEKQVYDAH.
Post Translational Modifications Ubiquitinated. Undergo monoubiquitination and multi- and/or polyubiquitination. Monoubiquitination of N-terminal lysines promotes integration in a ternary complex with UBXN6 and VCP which promotes oligomeric CAV1 targeting to lysosomes for degradation. Ubiquitinated by ZNRF1.leading to degradation and modulation of the TLR4-mediated immune response. The initiator methionine for isoform 2 is removed during or just after translation. The new N-terminal amino acid is then N-acetylated. Phosphorylated at Tyr-14 by ABL1 in response to oxidative stress.
Function May act as a scaffolding protein within caveolar membranes. Forms a stable heterooligomeric complex with CAV2 that targets to lipid rafts and drives caveolae formation. Mediates the recruitment of CAVIN proteins (CAVIN1/2/3/4) to the caveolae. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. Negatively regulates TGFB1-mediated activation of SMAD2/3 by mediating the internalization of TGFBR1 from membrane rafts leading to its subsequent degradation.
Protein Name Caveolin-1
Database Links Reactome: R-HSA-163560
Reactome: R-HSA-203615
Reactome: R-HSA-203641
Reactome: R-HSA-210991
Reactome: R-HSA-4641262
Reactome: R-HSA-5218920
Reactome: R-HSA-8980692
Reactome: R-HSA-9009391
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013405
Reactome: R-HSA-9013406
Reactome: R-HSA-9013407
Reactome: R-HSA-9013408
Reactome: R-HSA-9013409
Reactome: R-HSA-9013423
Reactome: R-HSA-9035034
Reactome: R-HSA-9617828
Reactome: R-HSA-9696264
Reactome: R-HSA-9696270
Reactome: R-HSA-9696273
Reactome: R-HSA-9735871
Reactome: R-HSA-9755779
Cellular Localisation Golgi Apparatus Membrane
Peripheral Membrane Protein
Cell Membrane
Membrane
Caveola
Membrane Raft
Golgi Apparatus
Trans-Golgi Network
Colocalized With Dpp4 In Membrane Rafts
Potential Hairpin-Like Structure In The Membrane
Membrane Protein Of Caveolae
Alternative Antibody Names Anti-Caveolin-1 antibody
Anti-CAV1 antibody
Anti-CAV antibody

Information sourced from Uniprot.org

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