| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-CD40 ligand is suitable for use in Western Blot and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:1000-2000 ELISA 1:5000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CD40LG |
| Gene ID: | 959 |
| Uniprot ID: | CD40L_HUMAN |
| Specificity: | This antibody detects endogenous levels of Human CD40 ligand |
| Immunogen: | Synthesized peptide derived from human CD40 ligand |
| Tissue Specificity | Specifically expressed on activated CD4+ T-lymphocytes. |
| Post Translational Modifications | The soluble form derives from the membrane form by proteolytic processing. N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. Not O-glycosylated. |
| Function | Cytokine that acts as a ligand to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B. Induces the activation of kinases MAPK8 and PAK2 in T-cells. Induces tyrosine phosphorylation of isoform 3 of CD28. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching. CD40 ligand, soluble form: Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3.both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling. |
| Protein Name | Cd40 Ligand Cd40-L T-Cell Antigen Gp39 Tnf-Related Activation Protein Trap Tumor Necrosis Factor Ligand Superfamily Member 5 Cd Antigen Cd154 Cleaved Into - Cd40 Ligand - Membrane Form - Cd40 Ligand - Soluble Form Scd40l |
| Database Links | Reactome: R-HSA-198933 Reactome: R-HSA-5668541 Reactome: R-HSA-5676594 |
| Cellular Localisation | Cell Membrane Single-Pass Type Ii Membrane Protein Cell Surface Cd40 Ligand Soluble Form: Secreted Release Of Soluble Cd40l From Platelets Is Partially Regulated By Gp Iib/Iiia Actin Polymerization And A Matrix Metalloproteinases (Mmp) Inhibitor-Sensitive Pathway |
| Alternative Antibody Names | Anti-Cd40 Ligand antibody Anti-Cd40-L antibody Anti-T-Cell Antigen Gp39 antibody Anti-Tnf-Related Activation Protein antibody Anti-Trap antibody Anti-Tumor Necrosis Factor Ligand Superfamily Member 5 antibody Anti-Cd Antigen Cd154 Cleaved Into - Cd40 Ligand - Membrane Form - Cd40 Ligand - Soluble Form antibody Anti-Scd40l antibody Anti-CD40LG antibody Anti-CD40L antibody Anti-TNFSF5 antibody Anti-TRAP antibody |
Information sourced from Uniprot.org