• Western blot analysis of extracts of various cell lines, using CHRNB1 antibody (STJ27248) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CHRNB1 antibody (25-235) (STJ27248)

SKU:
STJ27248

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CHRNB1 (25-235) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHRNB1
Gene ID: 1140
Uniprot ID: ACHB_HUMAN
Immunogen Region: 25-235
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-235 of human CHRNB1 (NP_000738.2).
Immunogen Sequence: EAEGRLREKLFSGYDSSVRP AREVGDRVRVSVGLILAQLI SLNEKDEEMSTKVYLDLEWT DYRLSWDPAEHDGIDSLRIT AESVWLPDVVLLNNNDGNFD VALDISVVVSSDGSVRWQPP GIYRSSCSIQVTYFPFDWQN CTMVFSSYSYDSSEVSLQTG LGPDGQGHQEIHIHEGTFIE NGQWEIIHKPSRLIQPPGDP RGGREGQRQEV
Function After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Protein Name Acetylcholine Receptor Subunit Beta
Cellular Localisation Postsynaptic Cell Membrane
Multi-Pass Membrane Protein
Cell Membrane
Alternative Antibody Names Anti-Acetylcholine Receptor Subunit Beta antibody
Anti-CHRNB1 antibody
Anti-ACHRB antibody
Anti-CHRNB antibody

Information sourced from Uniprot.org

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