Anti-Cleaved-Caspase-9 p35 (D315) antibody

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Product name Anti-Cleaved-Caspase-9 p35 (D315) antibody
Short Description Rabbit polyclonal against Cleaved-Caspase-9 p35 (D315)
Description Caspase-9 is a protein encoded by the CASP9 gene which is approximately 46, 2 kDa. Caspase-9 is localised to the cytosol, mitochondrion and nucleus. It is involved in the TNFR1 pathway, RET signalling and apoptosis modulation and signalling. Caspase-9 exist as an inactive proenzyme which undergoes proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. It is involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Caspase-9 is ubiquitously expressed with highest expression in the heart. Mutations in the CASP9 gene may result in cerebral hypoxia and cervical cancer. STJ90011 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of fragment activated Caspase-9 p35 protein resulting from cleavage adjacent to D315.
Applications ELISA, IF, IHC-p, WB
Dilution range WB 1:500-2000
IHC-p 1:50-300
IF 1:50-300
Specificity Cleaved-Caspase-9 p35 (D315) polyclonal antibody detects endogenous levels of fragment of activated Caspase-9 p35 protein resulting from cleavage adjacent to D315.
Protein Name Caspase-9
Casp-9
Apoptotic Protease Mch-6
Apoptotic Protease-Activating Factor 3
Apaf-3
Ice-Like Apoptotic Protease 6
Ice-Lap6 [Cleaved Into-Caspase-9 Subunit P35-Caspase-9 Subunit P10]
Immunogen Synthesized peptide derived from human Caspase-9 p35
Immunogen Region 240-320 aa, Internal
Storage Instruction Store at-20°C, and avoid repeat freeze-thaw cycles.
Note FOR RESEARCH USE ONLY (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 842
Gene Symbol CASP9
Molecular Weight 35 kDa
Database Links HGNC:1511
OMIM:602234
Alternative Names Anti-Caspase-9 antibody
Anti-Casp-9 antibody
Anti-Apoptotic Protease Mch-6 antibody
Anti-Apoptotic Protease-Activating Factor 3 antibody
Anti-Apaf-3 antibody
Anti-Ice-Like Apoptotic Protease 6 antibody
Anti-Ice-Lap6 [Cleaved Into-Caspase-9 Subunit P35-Caspase-9 Subunit P10] antibody
Anti-CASP9 MCH6 antibody
Function Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
Post-translational Modifications Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and-10 can also be involved in these processing events.; Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl; occurs in the response of cells to DNA damage.
Tissue Specificity Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
Swiss-Prot Key CASP9_HUMAN
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