| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-COL18A1 (1050-1150) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
COL18A1 |
| Gene ID: |
80781 |
| Uniprot ID: |
COIA1_HUMAN |
| Immunogen Region: |
1050-1150 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 1050-1150 of human COL18A1 (NP_085059.2). |
| Immunogen Sequence: |
PGLPGNQGPPGPKGAKGEVG PPGPPGQFPFDFLQLEAEMK GEKGDRGDAGQKGERGEPGG GGFFGSSLPGPPGPPGPRGY PGIPGPKGESIRGQPGPPGP Q |
| Tissue Specificity | Detected in placenta (at protein level). Present in multiple organs with highest levels in liver, lung and kidney. |
| Post Translational Modifications | Prolines at the third position of the tripeptide repeating unit (G-X-Y) of the triple-helical regions are hydroxylated. Circulating endostatins are found as sialoglycoprotein and asialoglycoprotein structures. Undergoes proteolytic processing by CTSL/cathepsin-L and elastase-like proteases to generate both non-collagenous domain 1 trimers and endostatin monomers. In tissue extracts (brain, skeletal muscle, heart, kidney, testis and liver) predominantly bands of approximately 38 kDa are detected.recombinant non-collagenous domain 1 shows similar mobility. In vitro, several proteolytic cleavage sites in the non-collagenous domain 1 hinge region generating different endostatin-like peptides are reported. |
| Function | Probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Non-collagenous domain 1: May regulate extracellular matrix-dependent motility and morphogenesis of endothelial and non-endothelial cells.the function requires homotrimerization and implicates MAPK signaling. Endostatin: Potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling. Inhibits VEGFA-induced endothelial cell proliferation and migration. Seems to inhibit VEGFA-mediated signaling by blocking the interaction of VEGFA to its receptor KDR/VEGFR2. Modulates endothelial cell migration in an integrin-dependent manner implicating integrin ITGA5:ITGB1 and to a lesser extent ITGAV:ITGB3 and ITGAV:ITGB5. May negatively regulate the activity of homotrimeric non-collagenous domain 1. |
| Protein Name | Collagen Alpha-1(Xviii Chain Cleaved Into - Endostatin - Non-Collagenous Domain 1Nc1 |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1592389Reactome: R-HSA-1650814Reactome: R-HSA-2022090Reactome: R-HSA-216083Reactome: R-HSA-3000157Reactome: R-HSA-8948216 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixBasement MembraneNon-Collagenous Domain 1: SecretedEndostatin: Secreted |
| Alternative Antibody Names | Anti-Collagen Alpha-1(Xviii Chain Cleaved Into - Endostatin - Non-Collagenous Domain 1 antibodyAnti-Nc1 antibodyAnti-COL18A1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
