Anti-DARS antibody (222-501) (STJ28657)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal DARS (222-501) antibody for use in WB, IHC-P, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DARS1 |
| Gene ID | 1615 |
| Uniprot ID | SYDC_HUMAN |
| Immunogen Region | 222-501 |
| Immunogen Sequence | KIISAASEGGANVFTVSYFK NNAYLAQSPQLYKQMCICAD FEKVFSIGPVFRAEDSNTHR HLTEFVGLDIEMAFNYHYHE VMEEIADTMVQIFKGLQERF QTEIQTVNKQFPCEPFKFLE PTLRLEYCEALAMLREAGVE MGDEDDLSTPNEKLLGHLVK EKYDTDFYILDKYPLAVRPF YTMPDPRNPKQSNSYDMFMR GEEILSGAQRIHDPQLLTER ALHHGIDLEKIKAYIDSFR |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 222-501 of human DARS (NP_001340.2). |
Additional Info
| Tissue Specificity | Expression in the developing and adult brain shows similar patterns. Highly expressed in the ventricular and subventricular zones, including hippocampal subfields, the midlateral temporal cortex and the frontal polar cortex. The cerebellum, cerebral cortex, hippocampus, and lateral ventricle show preferential neuronal expression. Expression in the peripheral neurons is evident in the colon. |
| Function | Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. |
| Protein Name | Aspartate--Trna Ligase - CytoplasmicAspartyl-Trna SynthetaseAsprsCell Proliferation-Inducing Gene 40 Protein |
| Database Links | Reactome: R-HSA-2408522Reactome: R-HSA-379716Reactome: R-HSA-9856649 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Aspartate--Trna Ligase - Cytoplasmic antibodyAnti-Aspartyl-Trna Synthetase antibodyAnti-Asprs antibodyAnti-Cell Proliferation-Inducing Gene 40 Protein antibodyAnti-DARS1 antibodyAnti-DARS antibodyAnti-PIG40 antibody |
Information sourced from Uniprot.org