| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DCLRE1A (1-300) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DCLRE1A |
| Gene ID: |
9937 |
| Uniprot ID: |
DCR1A_HUMAN |
| Immunogen Region: |
1-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DCLRE1A (NP_001258745.1). |
| Immunogen Sequence: |
MLEDISEEDIWEYKSKRKPK RVDPNNGSKNILKSVEKATD GKYQSKRSRNRKRAAEAKEV KDHEVPLGNAGCQTSVASSQ NSSCGDGIQQTQDKETTPGK LCRTQKSQHVSPKIRPVYDG YCPNCQMPFSSLIGQTPRWH VFECLDSPPRSETECPDGLL CTSTIPFHYKRYTHFLLAQS RAGDHPFSSPSPASGGSFSE TKSGVLCSLEERWSSYQNQT DNSVSNDPLLMTQYFKKSP |
| Tissue Specificity | Expressed in brain, heart, kidney, liver, pancreas, placenta and skeletal muscle. |
| Function | May be required for DNA interstrand cross-link repair. Also required for checkpoint mediated cell cycle arrest in early prophase in response to mitotic spindle poisons. Possesses beta-lactamase activity, catalyzing the hydrolysis of penicillin G and nitrocefin. Exhibits no activity towards other beta-lactam antibiotic classes including cephalosporins (cefotaxime) and carbapenems (imipenem). |
| Protein Name | Dna Cross-Link Repair 1a ProteinBeta-Lactamase Dclre1aSnm1 Homolog AHsnm1Hsnm1a |
| Database Links | Reactome: R-HSA-6783310 |
| Cellular Localisation | NucleusIn Some Cells It May Be Found In Typically 1 Or 2 Discrete Nuclear Aggregates Of Unknown Function Which Also Contain Tp53bp1Also Found In Multiple Discrete Nuclear Foci Which Increase In Number Following Treatment With Ionizing Radiation Or Interstrand Cross-Linking AgentsThese Foci Overlap With Those Formed By The Mrn Complex (Composed Of Mre11Rad50 And Nbn) And Brca1 |
| Alternative Antibody Names | Anti-Dna Cross-Link Repair 1a Protein antibodyAnti-Beta-Lactamase Dclre1a antibodyAnti-Snm1 Homolog A antibodyAnti-Hsnm1 antibodyAnti-Hsnm1a antibodyAnti-DCLRE1A antibodyAnti-KIAA0086 antibodyAnti-SNM1 antibodyAnti-SNM1A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89098/357557/STJ11100026_1__69013.1713121681.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
