| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DLG4 (420-510) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DLG4 |
| Gene ID: |
1742 |
| Uniprot ID: |
DLG4_HUMAN |
| Immunogen Region: |
420-510 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 420-510 of human PSD95 (NP_001308004.1). |
| Immunogen Sequence: |
TASLRSNPKRGFYIRALFDY DKTKDCGFLSQALSFRFGDV LHVIDASDEEWWQARRVHSD SETDDIGFIPSKRRVERREW SRLKAKDWGSS |
| Tissue Specificity | Brain. |
| Post Translational Modifications | Palmitoylated. Palmitoylation is required for targeting to postsynaptic density, plasma membrane and synapses. Palmitoylation by ZDHHC2 occurs when the synaptic activity decreases and induces DLG4 synaptic clustering. Palmitoylation by ZDHHC15 regulates trafficking to the postsynaptic density and function in synaptogenesis. Palmitoylation may play a role in glutamate receptor GRIA1 synapse clustering. Depalmitoylated by ABHD17A and ABHD17B and to a lesser extent by ABHD17C, ABHD12, ABHD13, LYPLA1 and LYPLA2. Undergoes rapid synaptic palmitoylation/depalmitoylation cycles during neuronal development which slow down in mature neurons. Ubiquitinated by MDM2 in response to NMDA receptor activation, leading to proteasome-mediated degradation of DLG4 which is required for AMPA receptor endocytosis. |
| Function | Postsynaptic scaffolding protein that plays a critical role in synaptogenesis and synaptic plasticity by providing a platform for the postsynaptic clustering of crucial synaptic proteins. Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. Also regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression. Under basal conditions, cooperates with FYN to stabilize palmitoyltransferase ZDHHC5 at the synaptic membrane through FYN-mediated phosphorylation of ZDHHC5 and its subsequent inhibition of association with endocytic proteins. |
| Protein Name | Disks Large Homolog 4Postsynaptic Density Protein 95Psd-95Synapse-Associated Protein 90Sap-90Sap90 |
| Database Links | Reactome: R-HSA-1236394Reactome: R-HSA-399719Reactome: R-HSA-438066Reactome: R-HSA-442982Reactome: R-HSA-447038Reactome: R-HSA-451308Reactome: R-HSA-5625900Reactome: R-HSA-5673001Reactome: R-HSA-5682910Reactome: R-HSA-6794361Reactome: R-HSA-8849932Reactome: R-HSA-9609736Reactome: R-HSA-9617324Reactome: R-HSA-9620244 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic SidePostsynaptic DensitySynapseCytoplasmCell ProjectionAxonDendritic SpineDendritePresynapseHigh Levels In Postsynaptic Density Of Neurons In The ForebrainAlso In Presynaptic Region Of Inhibitory Synapses Formed By Cerebellar Basket Cells On Axon Hillocks Of Purkinje CellsSuppression Of Neuronal Activity Induces Synaptic Accumulation And Clustering Of Dlg4 |
| Alternative Antibody Names | Anti-Disks Large Homolog 4 antibodyAnti-Postsynaptic Density Protein 95 antibodyAnti-Psd-95 antibodyAnti-Synapse-Associated Protein 90 antibodyAnti-Sap-90 antibodyAnti-Sap90 antibodyAnti-DLG4 antibodyAnti-PSD95 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
