Anti-DNASE1 antibody (22-282) (STJ23404)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal DNASE1 (22-282) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DNASE1 |
| Gene ID | 1773 |
| Uniprot ID | DNAS1_HUMAN |
| Immunogen Region | 22-282 |
| Immunogen Sequence | SLKIAAFNIQTFGETKMSNA TLVSYIVQILSRYDIALVQE VRDSHLTAVGKLLDNLNQDA PDTYHYVVSEPLGRNSYKER YLFVYRPDQVSAVDSYYYDD GCEPCGNDTFNREPAIVRFF SRFTEVREFAIVPLHAAPGD AVAEIDALYDVYLDVQEKWG LEDVMLMGDFNAGCSYVRPS QWSSIRLWTSPTFQWLIPDS ADTTATPTHCAYDRIVVAGM LLRGAVVPDSALPFNFQAA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 22-282 of human DNASE1 (NP_005214.2). |
Additional Info
| Tissue Specificity | Principally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva. |
| Function | Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs. Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA. Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation. |
| Protein Name | Deoxyribonuclease-1Deoxyribonuclease IDnase IDornase Alfa |
| Database Links | |
| Cellular Localisation | SecretedZymogen GranuleNucleus EnvelopeSecretory ProteinStored In Zymogen Granules And Found In The Nuclear Envelope |
| Alternative Antibody Names | Anti-Deoxyribonuclease-1 antibodyAnti-Deoxyribonuclease I antibodyAnti-Dnase I antibodyAnti-Dornase Alfa antibodyAnti-DNASE1 antibodyAnti-DNL1 antibodyAnti-DRNI antibody |
Information sourced from Uniprot.org