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Anti-DRP1 antibody

[STJ92779] Download PDF Print Data Sheet

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Product name Anti-DRP1 antibody
Short Description Rabbit polyclonal to DRP1.
Description DRP1 is a protein encoded by the DNM1L gene which is approximately 81,8 kDa. DRP1 is localised to the cytoplasm and Golgi apparatus. It is involved in toll-like receptor signalling pathways, the TNF signalling pathway, cytoskeletal signalling and apoptotic cleavage of cellular proteins. This protein falls under the dynamin superfamily of GTPases. It mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. DRP1 is expressed ubiquitously with the highest levels found in skeletal muscles, heart, kidney and brain. Mutations in the DNM1L gene may result in encephalopathy. STJ92779 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of DRP1 protein.
Applications ELISA, IHC, WB
Dilution range WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Specificity DRP1 Polyclonal Antibody detects endogenous levels of DRP1 protein.
Protein Name Dynamin-1-like protein
Dnm1p/Vps1p-like protein
DVLP
Dynamin family member proline-rich carboxyl-terminal domain less
Dymple
Dynamin-like protein
Dynamin-like protein 4
Dynamin-like protein IV
HdynIV
Dynamin-related protein 1
Immunogen Synthesized peptide derived from human DRP1 around the non-phosphorylation site of S637.
Immunogen Region 580-660 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 10059
Gene Symbol DNM1L
Molecular Weight 80 kDa
Database Links HGNC:2973
OMIM:603850
Alternative Names Dynamin-1-like protein antibody
Dnm1p/Vps1p-like protein antibody
DVLP antibody
Dynamin family member proline-rich carboxyl-terminal domain less antibody
Dymple antibody
Dynamin-like protein antibody
Dynamin-like protein 4 antibody
Dynamin-like protein IV antibody
HdynIV antibody
Dynamin-related protein 1 antibody
DLP1 antibody
Function Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Through its function in mitochondrial division, ensures the survival of at least some types of postmitotic neurons, including Purkinje cells, by suppressing oxidative damage. Required for normal brain development, including that of cerebellum. Facilitates developmentally regulated apoptosis during neural tube formation. Required for a normal rate of cytochrome c release and caspase activation during apoptosis; this requirement may depend upon the cell type and the physiological apoptotic cues. Plays an important role in mitochondrial fission during mitosis . Required for formation of endocytic vesicles. Proposed to regulate synaptic vesicle membrane dynamics through association with BCL2L1 isoform Bcl-X(L) which stimulates its GTPase activity in synaptic vesicles; the function may require its recruitment by MFF to clathrin-containing vesicles. Required for programmed necrosis execution. Isoform 1: Inhibits peroxisomal division when overexpressed. Isoform 4: Inhibits peroxisomal division when overexpressed.
Post-translational Modifications Phosphorylation/dephosphorylation events on two sites near the GED domain regulate mitochondrial fission. Phosphorylation on Ser-637 inhibits the GTPase activity, leading to a defect in mitochondrial fission promoting mitochondrial elongation. Dephosphorylated on this site by PPP3CA which promotes mitochondrial fission. Phosphorylation on Ser-616 activates the GTPase activity and promotes mitochondrial fission. Sumoylated on various lysine residues within the B domain, probably by MUL1. Sumoylation positively regulates mitochondrial fission. Desumoylated by SENP5 during G2/M transition of mitosis. Appears to be linked to its catalytic activity. S-nitrosylation increases DNM1L dimerization, mitochondrial fission and causes neuronal damage. Ubiquitination by MARCH5 affects mitochondrial morphology. O-GlcNAcylation augments the level of the GTP-bound active form of DRP1 and induces translocation from the cytoplasm to mitochondria in cardiomyocytes. It also decreases phosphorylation at Ser-637 .
Cellular Localization Cytoplasm, cytosol. Golgi apparatus. Endomembrane system. Peripheral membrane protein. Mitochondrion outer membrane Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Mainly cytosolic. Translocated to the mitochondrial membrane through O-GlcNAcylation and interaction with FIS1. Recruited to the mitochondrial outer membrane by interaction with MIEF1. Colocalized with MARCH5 at mitochondrial membrane. Localizes to mitochondria at sites of division. Localizes to mitochondria following necrosis induction. Associated with peroxisomal membranes, partly recruited there by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear. In some cell types, localizes to the Golgi complex. Binds to phospholipid membranes.
Tissue Specificity Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons.
Sequence and Domain Family The GED domain folds back to interact, in cis, with the GTP-binding domain and middle domain, and interacts, in trans, with the GED domains of other DNM1L molecules, and is thus critical for activating GTPase activity and for DNM1L dimerization.
Swiss-Prot Key O00429_HUMAN
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