• Western blot analysis of extracts of various cell lines, using ELN antibody (STJ23529) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of human lung cells using ELN rabbit polyclonal antibody (STJ23529) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-ELN antibody (27-200) (STJ23529)

SKU:
STJ23529

£169.50 - £449.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ELN (27-200) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ELN
Gene ID: 2006
Uniprot ID: ELN_HUMAN
Immunogen Region: 27-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 27-200 of human ELN (NP_001265868.1).
Immunogen Sequence: GGVPGAIPGGVPGGVFYPGA GLGALGGGALGPGGKPLKPV PGGLAGAGLGAGLGAFPAVT FPGALVPGGVADAAAAYKAA KAGAGLGGVPGVGGLGVSAG AVVPQPGAGVKPGKVPGVGL PGVYPGGVLPGARFPGVGVL PGVPTGAGVKPKAPGVGGAF AGIPGVGPFGGPQP
Tissue Specificity Expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin.
Post Translational Modifications Elastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine. Hydroxylation on proline residues within the sequence motif, GXPG, is most likely 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
Function Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.
Protein Name Elastin
Tropoelastin
Database Links Reactome: R-HSA-1474228
Reactome: R-HSA-1566948
Reactome: R-HSA-2129379
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Extracellular Matrix Of Elastic Fibers
Alternative Antibody Names Anti-Elastin antibody
Anti-Tropoelastin antibody
Anti-ELN antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance