| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-EPHA8 (28-300) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
EPHA8 |
| Gene ID: |
2046 |
| Uniprot ID: |
EPHA8_HUMAN |
| Immunogen Region: |
28-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 28-300 of human EPHA8 (NP_001006944.1). |
| Immunogen Sequence: |
ARGEVNLLDTSTIHGDWGWL TYPAHGWDSINEVDESFQPI HTYQVCNVMSPNQNNWLRTS WVPRDGARRVYAEIKFTLRD CNSMPGVLGTCKETFNLYYL ESDRDLGASTQESQFLKIDT IAADESFTGADLGVRRLKLN TEVRSVGPLSKRGFYLAFQD IGACLAILSLRIYYKKCPAM VRNLAAFSEAVTGADSSSLV EVRGQCVRHSEERDTPKMYC SAEGEWLVPIGKCVCSAGY |
| Post Translational Modifications | Phosphorylated. Phosphorylation is stimulated upon binding of its ligands including EFNA2, EFNA3 and EFNA5. Autophosphorylation on Tyr-616 is critical for association with FYN. Autophosphorylation on Tyr-839 modulates tyrosine kinase activity. Ubiquitinated. Ubiquitination by CBL regulates the receptor stability and activity through proteasomal degradation. ANKS1A prevents ubiquitination and degradation. |
| Function | Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. The GPI-anchored ephrin-A EFNA2, EFNA3, and EFNA5 are able to activate EPHA8 through phosphorylation. With EFNA5 may regulate integrin-mediated cell adhesion and migration on fibronectin substrate but also neurite outgrowth. During development of the nervous system also plays a role in axon guidance. Downstream effectors of the EPHA8 signaling pathway include FYN which promotes cell adhesion upon activation by EPHA8 and the MAP kinases in the stimulation of neurite outgrowth. |
| Protein Name | Ephrin Type-A Receptor 8Eph- And Elk-Related KinaseEph-Like Kinase 3Ek3Hek3Tyrosine-Protein Kinase Receptor Eek |
| Database Links | Reactome: R-HSA-2682334Reactome: R-HSA-3928663Reactome: R-HSA-3928665 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell ProjectionEarly Endosome MembraneUndergoes Clathrin-Mediated Endocytosis Upon Efna5-Binding And Is Targeted To Early Endosomes |
| Alternative Antibody Names | Anti-Ephrin Type-A Receptor 8 antibodyAnti-Eph- And Elk-Related Kinase antibodyAnti-Eph-Like Kinase 3 antibodyAnti-Ek3 antibodyAnti-Hek3 antibodyAnti-Tyrosine-Protein Kinase Receptor Eek antibodyAnti-EPHA8 antibodyAnti-EEK antibodyAnti-HEK3 antibodyAnti-KIAA1459 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
