| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | FICD |
| Gene ID: | 11153 |
| Uniprot ID: | FICD_HUMAN |
| Immunogen Region: | 119-458 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 119-458 of human FICD (NP_009007.2). |
| Immunogen Sequence: | QGKREKAQKLFMHALKMDPD FVDALTEFGIFSEEDKDIIQ ADYLYTRALTISPYHEKALV NRDRTLPLVEEIDQRYFSII DSKVKKVMSIPKGNSALRRV MEETYYHHIYHTVAIEGNTL TLSEIRHILETRYAVPGKSL EEQNEVIGMHAAMKYINTTL VSRIGSVTISDVLEIHRRVL GYVDPVEAGRFRTTQVLVGH HIPPHPQDVEKQMQEFVQWL NSEEAMNLHPVEFAALAHY |
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Auto-AMPylated in vitro.it is unclear whether auto-AMPylation is relevant in vivo. N-glycosylated.predominantly glycosylated at Asn-275. |
| Function | Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context. The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place. Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP. In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at 'Thr-518', thereby inactivating it. In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at 'Thr-518', leading to restore HSPA5/BiP activity. Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates. |
| Protein Name | Protein Adenylyltransferase Ficd Ampylator Ficd De-Ampylase Ficd Fic Domain-Containing Protein Huntingtin Yeast Partner E Huntingtin-Interacting Protein 13 Hip-13 Huntingtin-Interacting Protein E |
| Cellular Localisation | Endoplasmic Reticulum Membrane Single-Pass Type Ii Membrane Protein |
| Alternative Antibody Names | Anti-Protein Adenylyltransferase Ficd antibody Anti-Ampylator Ficd antibody Anti-De-Ampylase Ficd antibody Anti-Fic Domain-Containing Protein antibody Anti-Huntingtin Yeast Partner E antibody Anti-Huntingtin-Interacting Protein 13 antibody Anti-Hip-13 antibody Anti-Huntingtin-Interacting Protein E antibody Anti-FICD antibody Anti-HIP13 antibody Anti-HYPE antibody Anti-UNQ3041 antibody Anti-PRO9857 antibody |
Information sourced from Uniprot.org