• Immunofluorescence analysis of U-2 OS cells using GANAB Rabbit polyclonal antibody (STJ115790) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using GANAB Rabbit polyclonal antibody (STJ115790) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using GANAB Rabbit polyclonal antibody (STJ115790) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using GANAB Rabbit polyclonal antibody (STJ115790) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GANAB antibody (1-260) (STJ115790)

SKU:
STJ115790

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GANAB (1-260) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GANAB
Gene ID: 23193
Uniprot ID: GANAB_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human GANAB (NP_001265123.1).
Immunogen Sequence: MTRFRIDELEPRRPRYRVPD VLVADPPIARLSVSGRDENS VELTMAEGPYKIILTARPFR LDLLEDRSLLLSVNARGLLE FEHQRAPRVSQGSKDPAEGD GAQPEETPRDGDKPEETQGK AEKDEPGAWEETFKTHSDSK PYGPMSVGLDFSLPGMEHVY GIPEHADNLRLKVTEGGEPY RLYNLDVFQYELYNPMALYG SVPVLLAHNPHRDLGIFWLN AAETWVDISSNTAGKTLFG
Tissue Specificity Detected in placenta. Isoform 1 and isoform 2 are expressed in the kidney and liver.
Function Catalytic subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. Required for PKD1/Polycystin-1 and PKD2/Polycystin-2 maturation and localization to the cell surface and cilia.
Protein Name Neutral Alpha-Glucosidase Ab
Alpha-Glucosidase 2
Glucosidase Ii Subunit Alpha
Database Links Reactome: R-HSA-532668
Reactome: R-HSA-901042
Reactome: R-HSA-9683686
Reactome: R-HSA-9694548
Cellular Localisation Endoplasmic Reticulum
Golgi Apparatus
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Neutral Alpha-Glucosidase Ab antibody
Anti-Alpha-Glucosidase 2 antibody
Anti-Glucosidase Ii Subunit Alpha antibody
Anti-GANAB antibody
Anti-G2AN antibody
Anti-KIAA0088 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance