• Western blot analysis of extracts of various cell lines, using GCC2 antibody (STJ115756) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry of paraffin-embedded human colon using GCC2 antibody (STJ115756) at dilution of 1:150 (40x lens).
  • Immunohistochemistry of paraffin-embedded human stomach using GCC2 antibody (STJ115756) at dilution of 1:150 (40x lens).
  • Immunohistochemistry of paraffin-embedded human appendix using GCC2 antibody (STJ115756) at dilution of 1:150 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse spleen using GCC2 antibody (STJ115756) at dilution of 1:150 (40x lens).

Anti-GCC2 antibody (1-300) (STJ115756)

SKU:
STJ115756

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GCC2 (1-300) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GCC2
Gene ID: 9648
Uniprot ID: GCC2_HUMAN
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GCC2 (NP_852118.1).
Immunogen Sequence: MEDLVQDGVASPATPGTGKS KLETLPKEDLIKFAKKQMML IQKAKSRCTELEKEIEELRS KPVTEGTGDIIKALTERLDA LLLEKAETEQQCLSLKKENI KMKQEVEDSVTKMGDAHKEL EQSHINYVKEIENLKNELMA VRSKYSEDKANLQKQLEEAM NTQLELSEQLKFQNNSEDNV KKLQEEIEKIRPGFEEQILY LQKQLDATTDEKKETVTQLQ NIIEANSQHYQKNINSLQE
Tissue Specificity Ubiquitous.
Function Golgin which probably tethers transport vesicles to the trans-Golgi network (TGN) and regulates vesicular transport between the endosomes and the Golgi. As a RAB9A effector it is involved in recycling of the mannose 6-phosphate receptor from the late endosomes to the TGN. May also play a role in transport between the recycling endosomes and the Golgi. Required for maintenance of the Golgi structure, it is involved in the biogenesis of noncentrosomal, Golgi-associated microtubules through recruitment of CLASP1 and CLASP2.
Protein Name Grip And Coiled-Coil Domain-Containing Protein 2
185 Kda Golgi Coiled-Coil Protein
Gcc185
Cll-Associated Antigen Kw-11
Ctcl Tumor Antigen Se1-1
Ran-Binding Protein 2-Like 4
Ranbp2l4
Renal Carcinoma Antigen Ny-Ren-53
Database Links Reactome: R-HSA-6811440
Reactome: R-HSA-9725370
Cellular Localisation Cytoplasm
Golgi Apparatus
Trans-Golgi Network Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Grip And Coiled-Coil Domain-Containing Protein 2 antibody
Anti-185 Kda Golgi Coiled-Coil Protein antibody
Anti-Gcc185 antibody
Anti-Cll-Associated Antigen Kw-11 antibody
Anti-Ctcl Tumor Antigen Se1-1 antibody
Anti-Ran-Binding Protein 2-Like 4 antibody
Anti-Ranbp2l4 antibody
Anti-Renal Carcinoma Antigen Ny-Ren-53 antibody
Anti-GCC2 antibody
Anti-KIAA0336 antibody
Anti-RANBP2L4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance