| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: | GPR183 |
| Gene ID: | 1880 |
| Uniprot ID: | GP183_HUMAN |
| Immunogen Region: | 300-361 aa |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 300-361 of human GPR183 (NP_004942.1). |
| Immunogen Sequence: | NCCMDPFIYFFACKGYKRKV MRMLKRQVSVSISSAVKSAP EENSREMTETQMMIHSKSSN GK |
| Tissue Specificity | Expressed abundantly in lymphoid tissues such as spleen and lymph node, and in B- and T-lymphocytes. Also highly expressed in lung, heart and gastrointestinal tract, and weakly expressed in the urogenital system and brain. Expressed in astrocytes. |
| Function | G-protein coupled receptor expressed in lymphocytes that acts as a chemotactic receptor for B-cells, T-cells, splenic dendritic cells, monocytes/macrophages and astrocytes. Receptor for oxysterol 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) and other related oxysterols. Mediates cell positioning and movement of a number of cells by binding the 7-alpha,25-OHC ligand that forms a chemotactic gradient. Binding of 7-alpha,25-OHC mediates the correct localization of B-cells during humoral immune responses. Guides B-cell movement along the B-cell zone-T-cell zone boundary and later to interfollicular and outer follicular regions. Its specific expression during B-cell maturation helps position B-cells appropriately for mounting T-dependent antibody responses. Collaborates with CXCR5 to mediate B-cell migration.probably by forming a heterodimer with CXCR5 that affects the interaction between of CXCL13 and CXCR5. Also acts as a chemotactic receptor for some T-cells upon binding to 7-alpha,25-OHC ligand. Promotes follicular helper T (Tfh) cells differentiation by positioning activated T-cells at the follicle-T-zone interface, promoting contact of newly activated CD4 T-cells with activated dendritic cells and exposing them to Tfh-cell-promoting inducible costimulator (ICOS) ligand. Expression in splenic dendritic cells is required for their homeostasis, localization and ability to induce B- and T-cell responses: GPR183 acts as a chemotactic receptor in dendritic cells that mediates the accumulation of CD4(+) dendritic cells in bridging channels. Regulates migration of astrocytes and is involved in communication between astrocytes and macrophages. Promotes osteoclast precursor migration to bone surfaces. Signals constitutively through G(i)-alpha, but not G(s)-alpha or G(q)-alpha. Signals constitutively also via MAPK1/3 (ERK1/2). |
| Protein Name | G-Protein Coupled Receptor 183 Epstein-Barr Virus-Induced G-Protein Coupled Receptor 2 Ebi2 Ebv-Induced G-Protein Coupled Receptor 2 Hebi2 |
| Database Links | Reactome: R-HSA-373076 Reactome: R-HSA-418594 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein |
| Alternative Antibody Names | Anti-G-Protein Coupled Receptor 183 antibody Anti-Epstein-Barr Virus-Induced G-Protein Coupled Receptor 2 antibody Anti-Ebi2 antibody Anti-Ebv-Induced G-Protein Coupled Receptor 2 antibody Anti-Hebi2 antibody Anti-GPR183 antibody Anti-EBI2 antibody |
Information sourced from Uniprot.org