| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-GRK5 (371-590) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
GRK5 |
| Gene ID: |
2869 |
| Uniprot ID: |
GRK5_HUMAN |
| Immunogen Region: |
371-590 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 371-590 of human GRK5 (NP_005299.1). |
| Immunogen Sequence: |
LGCLIYEMIEGQSPFRGRKE KVKREEVDRRVLETEEVYSH KFSEEAKSICKMLLTKDAKQ RLGCQEEGAAEVKRHPFFRN MNFKRLEAGMLDPPFVPDPR AVYCKDVLDIEQFSTVKGVN LDHTDDDFYSKFSTGSVSIP WQNEMIETECFKELNVFGPN GTLPPDLNRNHPPEPPKKGL LQRLFKRQHQNNSKSSPSSK TSFNHHINSNHVSSNSTGSS |
| Tissue Specificity | Highest levels in heart, placenta, lung > skeletal muscle > brain, liver, pancreas > kidney. |
| Post Translational Modifications | Autophosphorylated. Autophosphorylation may play a critical role in the regulation of GRK5 kinase activity. |
| Function | Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro). |
| Protein Name | G Protein-Coupled Receptor Kinase 5G Protein-Coupled Receptor Kinase Grk5 |
| Database Links | Reactome: R-HSA-416476Reactome: R-HSA-418555 |
| Cellular Localisation | CytoplasmNucleusCell MembranePeripheral Membrane ProteinPredominantly Localized At The Plasma MembraneTargeted To The Cell Surface Through The Interaction With PhospholipidsNucleus Localization Is Regulated In A Gpcr And Ca(2+)/Calmodulin-Dependent Fashion |
| Alternative Antibody Names | Anti-G Protein-Coupled Receptor Kinase 5 antibodyAnti-G Protein-Coupled Receptor Kinase Grk5 antibodyAnti-GRK5 antibodyAnti-GPRK5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
