| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: | HERC1 |
| Gene ID: | 8925 |
| Uniprot ID: | HERC1_HUMAN |
| Immunogen Region: | 1200-1500 aa |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1200-1500 of human HERC1 (NP_003913.3). |
| Immunogen Sequence: | ALSGNEEQKPFDYKLRPEIA VYVDLALGCSKEPARSLWIS MQDYAVSKDWDSATLSNESL LDTVSRFVLAALLKHTNLLS QACGESRYQPGKHLSEVYRC VYKVRSRLLACKNLELIQTR SSSRDRWISENQDSADVDPQ EHSFTRTIDEEAEMEEQAER DREEGHPEPEDEEEEREHEV MTAGKIFQCFLSAREVARSR DRDRMNSGAGSGARADDPPP QSQQERRVSTDLPEGQDVY |
| Tissue Specificity | Widely expressed. |
| Function | Involved in membrane trafficking via some guanine nucleotide exchange factor (GEF) activity and its ability to bind clathrin. Acts as a GEF for Arf and Rab, by exchanging bound GDP for free GTP. Binds phosphatidylinositol 4,5-bisphosphate, which is required for GEF activity. May also act as a E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. |
| Protein Name | Probable E3 Ubiquitin-Protein Ligase Herc1 Hect Domain And Rcc1-Like Domain-Containing Protein 1 Hect-Type E3 Ubiquitin Transferase Herc1 P532 P619 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | Membrane Peripheral Membrane Protein Cytoplasm Cytosol Golgi Apparatus Recruited Onto Actin-Rich Surface Protrusions |
| Alternative Antibody Names | Anti-Probable E3 Ubiquitin-Protein Ligase Herc1 antibody Anti-Hect Domain And Rcc1-Like Domain-Containing Protein 1 antibody Anti-Hect-Type E3 Ubiquitin Transferase Herc1 antibody Anti-P532 antibody Anti-P619 antibody Anti-HERC1 antibody |
Information sourced from Uniprot.org