• Western blot analysis of extracts of SKOV3 cells, using IDO1 rabbit polyclonal antibody antibody (STJ24203) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of A-549 cells, using IDO1 rabbit polyclonal antibody antibody (STJ24203) at 1:500 dilution. A-549 cells were treated by hIFN-Gamma (100 ng/mL) at 37 °C for 48 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of U2OS cells using IDO1 rabbit polyclonal antibody (STJ24203) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-IDO1 antibody (1-403) (STJ24203)

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STJ24203

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IDO1 (1-403) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IDO1
Gene ID: 3620
Uniprot ID: I23O1_HUMAN
Immunogen Region: 1-403
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-403 of human IDO1 (NP_002155.1).
Immunogen Sequence: MAHAMENSWTISKEYHIDEE VGFALPNPQENLPDFYNDWM FIAKHLPDLIESGQLRERVE KLNMLSIDHLTDHKSQRLAR LVLGCITMAYVWGKGHGDVR KVLPRNIAVPYCQLSKKLEL PPILVYADCVLANWKKKDPN KPLTYENMDVLFSFRDGDCS KGFFLVSLLVEIAAASAIKV IPTVFKAMQMQERDTLLKAL LEIASCLEKALQVFHQIHDH VNPKAFFSVLRIYLSGWKG
Tissue Specificity Expressed in mature dendritic cells located in lymphoid organs (including lymph nodes, spleen, tonsils, Peyers's patches, the gut lamina propria, and the thymic medulla), in some epithelial cells of the female genital tract, as well as in endothelial cells of term placenta and in lung parenchyma. Weakly or not expressed in most normal tissues, but mostly inducible in most tissues. Expressed in more than 50% of tumors, either by tumoral, stromal, or endothelial cells (expression in tumor is associated with a worse clinical outcome). Not overexpressed in tumor-draining lymph nodes.
Function Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway. Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses. Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells. Acts as a suppressor of anti-tumor immunity. Limits the growth of intracellular pathogens by depriving tryptophan. Protects the fetus from maternal immune rejection.
Protein Name Indoleamine 2 -3-Dioxygenase 1
Ido-1
Indoleamine-Pyrrole 2 -3-Dioxygenase
Database Links Reactome: R-HSA-71240
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Indoleamine 2 -3-Dioxygenase 1 antibody
Anti-Ido-1 antibody
Anti-Indoleamine-Pyrrole 2 -3-Dioxygenase antibody
Anti-IDO1 antibody
Anti-IDO antibody
Anti-INDO antibody

Information sourced from Uniprot.org

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