| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | IER5 |
| Gene ID: | 51278 |
| Uniprot ID: | IER5_HUMAN |
| Immunogen Region: | 200-300 |
| Immunogen Sequence: | APQPAEDEPPAPPAVCPRKR CAAGVGGGPAGCPAPGSTPL KKPRRNLEQPPSGGEDDDAE EMETGNVANLISIFGSSFSG LLRKSPGGGREEEEGEESGP E |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human IER5 (NP_057629.2). |
| Tissue Specificity | Expressed in acute myeloid leukemia (AML) cells. |
| Function | Plays a role as a transcription factor. Mediates positive transcriptional regulation of several chaperone genes during the heat shock response in a HSF1-dependent manner. Mediates negative transcriptional regulation of CDC25B expression. Plays a role in the dephosphorylation of the heat shock factor HSF1 and ribosomal protein S6 kinase (S6K) by the protein phosphatase PP2A. Involved in the regulation of cell proliferation and resistance to thermal stress. Involved in the cell cycle checkpoint and survival in response to ionizing radiation. Associates with chromatin to the CDC25B promoter. |
| Protein Name | Immediate Early Response Gene 5 Protein |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmPredominantly CytoplasmicTranslocated In The Nucleus During Heat Shock |
| Alternative Antibody Names | Anti-Immediate Early Response Gene 5 Protein antibodyAnti-IER5 antibodyAnti-PP4583 antibodyAnti-SBBI48 antibody |
Information sourced from Uniprot.org
