• Western blot analysis of extracts of various cell lines, using IFT57 antibody (STJ29271) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IFT57 antibody (170-429) (STJ29271)

SKU:
STJ29271

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IFT57 (170-429) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IFT57
Gene ID: 55081
Uniprot ID: IFT57_HUMAN
Immunogen Region: 170-429
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 170-429 of human IFT57 (NP_060480.1).
Immunogen Sequence: VEELEEESVAEDDAELTLNK VDEEFVEEETDNEENFIDLN VLKAQTYHLDMNETAKQEDI LESTTDAAEWSLEVERVLPQ LKVTIRTDNKDWRIHVDQMH QHRSGIESALKETKGFLDKL HNEITRTLEKISSREKYINN QLENLVQEYRAAQAQLSEAK ERYQQGNGGVTERTRLLSEV MEELEKVKQEMEEKGSSMTD GAPLVKIKQSLTKLKQETVE MDIRIGIVEHTLLQSKLKE
Tissue Specificity Present in many tissues such as brain, thymus, lymph node, lung, liver, skin and kidney (at protein level).
Function Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway. Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator.however the relevance of such function remains unclear.
Protein Name Intraflagellar Transport Protein 57 Homolog
Dermal Papilla-Derived Protein 8
Estrogen-Related Receptor Beta-Like Protein 1
Hip1-Interacting Protein
Mhs4r2
Database Links Reactome: R-HSA-5610787
Reactome: R-HSA-5620924
Cellular Localisation Cell Projection
Cilium
Cytoplasm
Cytoskeleton
Cilium Basal Body
Concentrates Within The Inner Segment Of Cilia
Alternative Antibody Names Anti-Intraflagellar Transport Protein 57 Homolog antibody
Anti-Dermal Papilla-Derived Protein 8 antibody
Anti-Estrogen-Related Receptor Beta-Like Protein 1 antibody
Anti-Hip1-Interacting Protein antibody
Anti-Mhs4r2 antibody
Anti-IFT57 antibody
Anti-DERP8 antibody
Anti-ESRRBL1 antibody
Anti-HIPPI antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance