• Immunohistochemistry analysis of paraffin-embedded mouse kidney using IL3RA antibody (STJ24185) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using IL3RA antibody (STJ24185) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of A-549 cells, using IL3RA antibody (STJ24185) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IL3RA antibody (1-100) (STJ24185)

SKU:
STJ24185

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IL3RA (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IL3RA
Gene ID: 3563
Uniprot ID: IL3RA_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human IL3RA (NP_002174.1).
Immunogen Sequence: MVLLWLTLLLIALPCLLQTK EDPNPPITNLRMKAKAQQLT WDLNRNVTDIECVKDADYSM PAVNNSYCQFGAISLCEVTN YTVRVANPPFSTWILFPENS
Post Translational Modifications Ubiquitinated by RNFT2 in response to IL3. Ubiquitination leads ligand-induced degradation by the proteasome.
Function Cell surface receptor for IL3 expressed on hematopoietic progenitor cells, monocytes and B-lymphocytes that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Ligand stimulation rapidly induces hetrodimerization with IL3RB, phosphorylation and enzyme activity of effector proteins such as JAK2 and PI3K that play a role in signaling cell proliferation and differentiation. Activation of JAK2 leads to STAT5-mediated transcriptional program.
Protein Name Interleukin-3 Receptor Subunit Alpha
Il-3 Receptor Subunit Alpha
Il-3r Subunit Alpha
Il-3r-Alpha
Il-3ra
Cd Antigen Cd123
Database Links Reactome: R-HSA-512988
Reactome: R-HSA-5673001
Reactome: R-HSA-912526
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Interleukin-3 Receptor Subunit Alpha antibody
Anti-Il-3 Receptor Subunit Alpha antibody
Anti-Il-3r Subunit Alpha antibody
Anti-Il-3r-Alpha antibody
Anti-Il-3ra antibody
Anti-Cd Antigen Cd123 antibody
Anti-IL3RA antibody
Anti-IL3R antibody

Information sourced from Uniprot.org

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