• Western blot analysis of extracts of various cell lines, using IL5 antibody (STJ110003) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 15s.

Anti-IL5 antibody (20-134) (STJ110003)

SKU:
STJ110003

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IL5 (20-134) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IL5
Gene ID: 3567
Uniprot ID: IL5_HUMAN
Immunogen Region: 20-134
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-134 of human IL5 (NP_000870.1).
Immunogen Sequence: IPTEIPTSALVKETLALLST HRTLLIANETLRIPVPVHKN HQLCTEEIFQGIGTLESQTV QGGTVERLFKNLSLIKKYID GQKKKCGEERRRVNQFLDYL QEFLGVMNTEWIIES
Function Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. Acts also on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation. Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively.
Protein Name Interleukin-5
Il-5
B-Cell Differentiation Factor I
Eosinophil Differentiation Factor
T-Cell Replacing Factor
Trf
Database Links Reactome: R-HSA-512988
Reactome: R-HSA-5673001
Reactome: R-HSA-912526
Cellular Localisation Secreted
Alternative Antibody Names Anti-Interleukin-5 antibody
Anti-Il-5 antibody
Anti-B-Cell Differentiation Factor I antibody
Anti-Eosinophil Differentiation Factor antibody
Anti-T-Cell Replacing Factor antibody
Anti-Trf antibody
Anti-IL5 antibody

Information sourced from Uniprot.org

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