• Western blot analysis of extracts of various cell lines, using IL5RA antibody (STJ111288) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using IL5RA antibody (STJ111288) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-IL5RA antibody (20-335) (STJ111288)

SKU:
STJ111288

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IL5RA (20-335) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IL5RA
Gene ID: 3568
Uniprot ID: IL5RA_HUMAN
Immunogen Region: 20-335
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-335 of human IL5RA (NP_783855.1).
Immunogen Sequence: ADLLPDEKISLLPPVNFTIK VTGLAQVLLQWKPNPDQEQR NVNLEYQVKINAPKEDDYET RITESKCVTILHKGFSASVR TILQNDHSLLASSWASAELH APPGSPGTSIVNLTCTTNTT EDNYSRLRSYQVSLHCTWLV GTDAPEDTQYFLYYRYGSWT EECQEYSKDTLGRNIACWFP RTFILSKGRDWLAVLVNGSS KHSAIRPFDQLFALHAIDQI NPPLNVTAEIEGTRLSIQW
Tissue Specificity Expressed on eosinophils and basophils.
Function Cell surface receptor that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. Acts by forming an heterodimeric receptor with CSF2RB subunit and subsequently binding to interleukin-5. In unstimulated conditions, interacts constitutively with JAK2. Heterodimeric receptor activation leads to JAK2 stimulation and subsequent activation of the JAK-STAT pathway.
Protein Name Interleukin-5 Receptor Subunit Alpha
Il-5 Receptor Subunit Alpha
Il-5r Subunit Alpha
Il-5r-Alpha
Il-5ra
Cdw125
Cd Antigen Cd125
Database Links Reactome: R-HSA-512988
Reactome: R-HSA-5673001
Reactome: R-HSA-912526
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Interleukin-5 Receptor Subunit Alpha antibody
Anti-Il-5 Receptor Subunit Alpha antibody
Anti-Il-5r Subunit Alpha antibody
Anti-Il-5r-Alpha antibody
Anti-Il-5ra antibody
Anti-Cdw125 antibody
Anti-Cd Antigen Cd125 antibody
Anti-IL5RA antibody
Anti-IL5R antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance