• Western blot analysis of various lysates, using LIF rabbit polyclonal antibody (STJ29846) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of LIF in paraffin-embedded human liver cancer tissue using LIF rabbit polyclonal antibody (STJ29846) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of LIF in paraffin-embedded mouse kidney tissue using LIF rabbit polyclonal antibody (STJ29846) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of LIF in paraffin-embedded rat liver tissue using LIF rabbit polyclonal antibody (STJ29846) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-LIF antibody (23-202) (STJ29846)

SKU:
STJ29846

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LIF (23-202) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LIF
Gene ID: 3976
Uniprot ID: LIF_HUMAN
Immunogen Region: 23-202
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 23-202 of human LIF (NP_002300.1).
Immunogen Sequence: SPLPITPVNATCAIRHPCHN NLMNQIRSQLAQLNGSANAL FILYYTAQGEPFPNNLDKLC GPNVTDFPPFHANGTEKAKL VELYRIVVYLGTSLGNITRD QKILNPSALSLHSKLNATAD ILRGLLSNVLCRLCSKYHVG HVDVTYGPDTSGKDVFQKKK LGCQLLGKYKQIIAVLAQAF
Function LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Protein Name Leukemia Inhibitory Factor
Lif
Differentiation-Stimulating Factor
D Factor
Melanoma-Derived Lpl Inhibitor
Mlpli
Emfilermin
Database Links Reactome: R-HSA-6783783
Reactome: R-HSA-6785807
Reactome: R-HSA-6788467
Cellular Localisation Secreted
Alternative Antibody Names Anti-Leukemia Inhibitory Factor antibody
Anti-Lif antibody
Anti-Differentiation-Stimulating Factor antibody
Anti-D Factor antibody
Anti-Melanoma-Derived Lpl Inhibitor antibody
Anti-Mlpli antibody
Anti-Emfilermin antibody
Anti-LIF antibody
Anti-HILDA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance