• Western blot analysis using LPL monoclonal antibody against HeLa cell lysate (1).

Anti-LPL antibody [2C5] (STJ98222)

SKU:
STJ98222

£139.00 - £336.00
Current Stock:
Host: Mouse
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Lipoprotein lipase is suitable for use in Western Blot and ELISA research applications.
Clonality: Monoclonal
Clone ID: 2C5
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LPL
Gene ID: 4023
Uniprot ID: LIPL_HUMAN
Specificity: LPL Monoclonal Antibody detects endogenous levels of LPL protein.
Immunogen: Purified recombinant fragment of LPL expressed in E. Coli.
Post Translational Modifications Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity.
Function Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage. Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity. Mediates margination of triglyceride-rich lipoprotein particles in capillaries. Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans.
Protein Name Lipoprotein Lipase
Lpl
Phospholipase A1
Database Links Reactome: R-HSA-381340
Reactome: R-HSA-8963889
Reactome: R-HSA-8963901
Reactome: R-HSA-975634
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Extracellular Side
Secreted
Extracellular Space
Extracellular Matrix
Newly Synthesized Lpl Binds To Cell Surface Heparan Proteoglycans And Is Then Released By Heparanase
Subsequently
It Becomes Attached To Heparan Proteoglycan On Endothelial Cells
Locates To The Plasma Membrane Of Microvilli Of Hepatocytes With Triglyceride-Rich Lipoproteins (Trl)
Some Of The Bound Lpl Is Then Internalized And Located Inside Non-Coated Endocytic Vesicles
Alternative Antibody Names Anti-Lipoprotein Lipase antibody
Anti-Lpl antibody
Anti-Phospholipase A1 antibody
Anti-LPL antibody
Anti-LIPD antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance