• Western blot analysis of extracts of various cell lines, using JNK3 antibody (STJ24483) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-MAPK10 antibody (175-464) (STJ24483)

SKU:
STJ24483

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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-JNK3 (175-464) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAPK10
Gene ID: 5602
Uniprot ID: MK10_HUMAN
Immunogen Region: 175-464
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 175-464 of human JNK3 (NP_620448.1).
Immunogen Sequence: CGIKHLHSAGIIHRDLKPSN IVVKSDCTLKILDFGLARTA GTSFMMTPYVVTRYYRAPEV ILGMGYKENVDIWSVGCIMG EMVRHKILFPGRDYIDQWNK VIEQLGTPCPEFMKKLQPTV RNYVENRPKYAGLTFPKLFP DSLFPADSEHNKLKASQARD LLSKMLVIDPAKRISVDDAL QHPYINVWYDPAEVEAPPPQ IYDKQLDEREHTIEEWKELI YKEVMNSEEKTKNGVVKGQ
Tissue Specificity Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney.
Post Translational Modifications Dually phosphorylated on Thr-221 and Tyr-223 by MAP2K4 and MAP2K7, which activates the enzyme. MAP2K7 shows a strong preference for Thr-221 while MAP2K4 phosphorylates Tyr-223 preferentially. Weakly autophosphorylated on threonine and tyrosine residues in vitro. Palmitoylation regulates subcellular location and axonal development.
Function Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the amyloid-beta precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the photic regulation of the circadian clock. Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1.
Protein Name Mitogen-Activated Protein Kinase 10
Map Kinase 10
Mapk 10
Map Kinase P49 3f12
Stress-Activated Protein Kinase 1b
Sapk1b
Stress-Activated Protein Kinase Jnk3
C-Jun N-Terminal Kinase 3
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-2871796
Reactome: R-HSA-450321
Reactome: R-HSA-450341
Cellular Localisation Cytoplasm
Membrane
Lipid-Anchor
Nucleus
Mitochondrion
Palmitoylation Regulates Mapk10 Trafficking To Cytoskeleton
Recruited To The Mitochondria In The Presence Of Sarm1
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase 10 antibody
Anti-Map Kinase 10 antibody
Anti-Mapk 10 antibody
Anti-Map Kinase P49 3f12 antibody
Anti-Stress-Activated Protein Kinase 1b antibody
Anti-Sapk1b antibody
Anti-Stress-Activated Protein Kinase Jnk3 antibody
Anti-C-Jun N-Terminal Kinase 3 antibody
Anti-MAPK10 antibody
Anti-JNK3 antibody
Anti-JNK3A antibody
Anti-PRKM10 antibody
Anti-SAPK1B antibody

Information sourced from Uniprot.org

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