Anti-MIF antibody

[STJ94126] Download PDF Print Data Sheet

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Product name Anti-MIF antibody
Short Description Rabbit polyclonal against MIF
Description MIF is a protein encoded by the MIF gene which is approximately 12,4 kDa. MIF is secreted and localised to the cytoplasm. It is involved in the innate immune system, tyrosine metabolism, PEDF induced signalling and cell surface interactions at the vascular wall. It is a pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens. It plays a role in the regulation of macrophage function in host defence through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signalling pathways. MIF is expressed in the lung, nervous system, skin, eye and blood. Mutations in the MIF gene may result in rheumatoid arthritis. STJ94126 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of MIF protein.
Applications ELISA, WB
Dilution range WB 1:500-1:2000
ELISA 1:10000
Specificity MIF Polyclonal Antibody detects endogenous levels of MIF protein.
Protein Name Macrophage migration inhibitory factor
MIF
Glycosylation-inhibiting factor
GIF
L-dopachrome isomerase
L-dopachrome tautomerase
Phenylpyruvate tautomerase
Immunogen Synthesized peptide derived from human MIF.
Immunogen Region Internal
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Validated Application IF IHC WB
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 4282
Gene Symbol MIF
Molecular Weight 12 kDa
Database Links HGNC:7097
OMIM:153620
Alternative Names Macrophage migration inhibitory factor
MIF
Glycosylation-inhibiting factor
GIF
L-dopachrome isomerase
L-dopachrome tautomerase
Phenylpyruvate tautomerase
Function Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
Cellular Localization Secreted Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
Swiss-Prot Key P14174_HUMAN
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