Anti-MKI67 antibody

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Product name Anti-MKI67 antibody
Short Description Rabbit polyclonal to Ki67
Applications IF, IHC, WB
Dilution range WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200
Protein Name Proliferation Marker Protein Ki-67
Antigen Identified By Monoclonal Antibody Ki-67
Antigen Ki-67
Antigen Ki67
Immunogen Recombinant protein of human Ki67
Storage Instruction Store at-20°C and avoid freeze-thaw cycles.
Note FOR RESEARCH USE ONLY (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Purification Affinity purification
Isotype IgG
Formulation PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Gene ID 4288
Gene Symbol MKI67
Molecular Weight Calculated: 319kDa/358kDa. Observed: 358kDa
Alternative Names Anti-Proliferation Marker Protein Ki-67 antibody
Anti-Antigen Identified By Monoclonal Antibody Ki-67 antibody
Anti-Antigen Ki-67 antibody
Anti-Antigen Ki67 antibody
Anti-MKI67 antibody
Function Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization. It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Post-translational Modifications Phosphorylated. Hyperphosphorylated in mitosis. Hyperphosphorylated form does not bind DNA.
Cellular Localization Chromosome
Nucleus
Nucleolus
Associates With The Surface Of The Mitotic Chromosome
The Perichromosomal Layer
And Covers A Substantial Fraction Of The Mitotic Chromosome Surface
Associates With Satellite Dna In G1 Phase
Binds Tightly To Chromatin In Interphase
Chromatin-Binding Decreases In Mitosis When It Associates With The Surface Of The Condensed Chromosomes
Predominantly Localized In The G1 Phase In The Perinucleolar Region
In The Later Phases It Is Also Detected Throughout The Nuclear Interior
Being Predominantly Localized In The Nuclear Matrix
Swiss-Prot Key KI67_HUMAN
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