| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Mixed lineage kinase domain-like protein is suitable for use in Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MLKL |
| Gene ID: | 197259 |
| Uniprot ID: | MLKL_HUMAN |
| Specificity: | This antibody detects endogenous levels of MLKL Phospho-ser345 at Human, Mouse, Rat |
| Immunogen: | Synthesized peptide derived from human MLKL Phospho-ser345 |
| Function | Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Does not have protein kinase activity. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following activation by ZBP1, MLKL is phosphorylated by RIPK3 in the nucleus, triggering disruption of the nuclear envelope and leakage of cellular DNA into the cytosol.following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol. Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function. |
| Protein Name | Mixed Lineage Kinase Domain-Like Protein Hmlkl |
| Database Links | Reactome: R-HSA-3295583 Reactome: R-HSA-5213460 Reactome: R-HSA-5675482 Reactome: R-HSA-9686347 |
| Cellular Localisation | Cytoplasm Cell Membrane Nucleus Localizes To The Cytoplasm And Translocates To The Plasma Membrane On Necroptosis Induction Localizes To The Nucleus In Response To Orthomyxoviruses Infection |
| Alternative Antibody Names | Anti-Mixed Lineage Kinase Domain-Like Protein antibody Anti-Hmlkl antibody Anti-MLKL antibody |
Information sourced from Uniprot.org