• Western blot analysis of extracts of various cell lines, using MTHFD1 antibody (STJ111371) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunofluorescence analysis of U-2 OS cells using MTHFD1 Rabbit polyclonal antibody (STJ111371) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using MTHFD1 rabbit polyclonal antibody (STJ111371) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using MTHFD1 antibody (STJ111371) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-MTHFD1 antibody (1-270) (STJ111371)

SKU:
STJ111371

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MTHFD1 (1-270) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MTHFD1
Gene ID: 4522
Uniprot ID: C1TC_HUMAN
Immunogen Region: 1-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human MTHFD1 (NP_005947.3).
Immunogen Sequence: MAPAEILNGKEISAQIRARL KNQVTQLKEQVPGFTPRLAI LQVGNRDDSNLYINVKLKAA EEIGIKATHIKLPRTTTESE VMKYITSLNEDSTVHGFLVQ LPLDSENSINTEEVINAIAP EKDVDGLTSINAGKLARGDL NDCFIPCTPKGCLELIKETG VPIAGRHAVVVGRSKIVGAP MHDLLLWNNATVTTCHSKTA HLDEEVNKGDILVVATGQPE MVKGEWIKPGAIVIDCGIN
Tissue Specificity Ubiquitous.
Function Trifunctional enzyme that catalyzes the interconversion of three forms of one-carbon-substituted tetrahydrofolate: (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate, 5,10-methenyltetrahydrofolate and (6S)-10-formyltetrahydrofolate. These derivatives of tetrahydrofolate are differentially required in nucleotide and amino acid biosynthesis, (6S)-10-formyltetrahydrofolate being required for purine biosynthesis while (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate is used for serine and methionine biosynthesis for instance.
Protein Name C-1-Tetrahydrofolate Synthase - Cytoplasmic
C1-Thf Synthase
Epididymis Secretory Sperm Binding Protein Cleaved Into - C-1-Tetrahydrofolate Synthase - Cytoplasmic - N-Terminally Processed Includes - Methylenetetrahydrofolate Dehydrogenase - Methenyltetrahydrofolate Cyclohydrolase - Formyltetrahydrofolate Synthetase
Database Links Reactome: R-HSA-196757
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-C-1-Tetrahydrofolate Synthase - Cytoplasmic antibody
Anti-C1-Thf Synthase antibody
Anti-Epididymis Secretory Sperm Binding Protein Cleaved Into - C-1-Tetrahydrofolate Synthase - Cytoplasmic - N-Terminally Processed Includes - Methylenetetrahydrofolate Dehydrogenase - Methenyltetrahydrofolate Cyclohydrolase - Formyltetrahydrofolate Synthetase antibody
Anti-MTHFD1 antibody
Anti-MTHFC antibody
Anti-MTHFD antibody

Information sourced from Uniprot.org

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