• Western blot analysis of extracts of various cell lines, using MTHFS antibody (STJ114643) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MTHFS antibody (1-203) (STJ114643)

SKU:
STJ114643

£169.50 - £449.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MTHFS (1-203) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MTHFS
Gene ID: 10588
Uniprot ID: MTHFS_HUMAN
Immunogen Region: 1-203
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-203 of human MTHFS (NP_006432.1).
Immunogen Sequence: MAAAAVSSAKRSLRGELKQR LRAMSAEERLRQSRVLSQKV IAHSEYQKSKRISIFLSMQD EIETEEIIKDIFQRGKICFI PRYRFQSNHMDMVRIESPEE ISLLPKTSWNIPQPGEGDVR EEALSTGGLDLIFMPGLGFD KHGNRLGRGKGYYDAYLKRC LQHQEVKPYTLALAFKEQIC LQVPVNENDMKVDEVLYEDS STA
Function Contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. Catalyzes the irreversible conversion of 5-formyltetrahydrofolate (5-FTHF) to yield 5,10-methenyltetrahydrofolate.
Protein Name 5-Formyltetrahydrofolate Cyclo-Ligase
5 -10-Methenyl-Tetrahydrofolate Synthetase
Mthfs
Methenyl-Thf Synthetase
Database Links Reactome: R-HSA-196757
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-5-Formyltetrahydrofolate Cyclo-Ligase antibody
Anti-5 -10-Methenyl-Tetrahydrofolate Synthetase antibody
Anti-Mthfs antibody
Anti-Methenyl-Thf Synthetase antibody
Anti-MTHFS antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance