| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NBN (520-754) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NBN |
| Gene ID: |
4683 |
| Uniprot ID: |
NBN_HUMAN |
| Immunogen Region: |
520-754 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 520-754 of human NBS1/NBN (NP_002476.2). |
| Immunogen Sequence: |
NNLFTDTDLKSIVKNSASKS HAAEKLRSNKKREMDDVAIE DEVLEQLFKDTKPELEIDVK VQKQEEDVNVRKRPRMDIET NDTFSDEAVPESSKISQENE IGKKRELKEDSLWSAKEISN NDKLQDDSEMLPKKLLLTEF RSLVIKNSTSRNPSGINDDY GQLKNFKKFKKVTYPGAGKL PHIIGGSDLIAHHARKNTEL EEWLRQEMEVQNQHAKEESL ADDLFRYNPYLKRRR |
| Tissue Specificity | Ubiquitous. Expressed at high levels in testis. |
| Post Translational Modifications | Phosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance. |
| Function | Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex. |
| Protein Name | NibrinCell Cycle Regulatory Protein P95Nijmegen Breakage Syndrome Protein 1 |
| Database Links | Reactome: R-HSA-2559586Reactome: R-HSA-5685938Reactome: R-HSA-5685939Reactome: R-HSA-5685942Reactome: R-HSA-5693548Reactome: R-HSA-5693554Reactome: R-HSA-5693565Reactome: R-HSA-5693568Reactome: R-HSA-5693571Reactome: R-HSA-5693579Reactome: R-HSA-5693607Reactome: R-HSA-5693616Reactome: R-HSA-6804756Reactome: R-HSA-69473Reactome: R-HSA-912446Reactome: R-HSA-9701192Reactome: R-HSA-9704331Reactome: R-HSA-9704646Reactome: R-HSA-9709570Reactome: R-HSA-9709603 |
| Cellular Localisation | NucleusPml BodyChromosomeTelomereLocalizes To Discrete Nuclear Foci After Treatment With Genotoxic AgentsAcetylation Of 'Lys-5' Of Histone H2ax (H2axk5ac) Promotes Nbn/Nbs1 Assembly At The Sites Of Dna Damage |
| Alternative Antibody Names | Anti-Nibrin antibodyAnti-Cell Cycle Regulatory Protein P95 antibodyAnti-Nijmegen Breakage Syndrome Protein 1 antibodyAnti-NBN antibodyAnti-NBS antibodyAnti-NBS1 antibodyAnti-P95 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
