| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NEFL |
| Gene ID: | 4747 |
| Uniprot ID: | NFL_HUMAN |
| Immunogen Region: | 60-250 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 60-250 of human Neurofilament L (NP_006149.2). |
| Immunogen Sequence: | SSGSLMPSLENLDLSQVAAI SNDLKSIRTQEKAQLQDLND RFASFIERVHELEQQNKVLE AELLVLRQKHSEPSRFRALY EQEIRDLRLAAEDATNEKQA LQGEREGLEETLRNLQARYE EEVLSREDAEGRLMEARKGA DEAALARAELEKRIDSLMDE ISFLKKVHEEEIAELQAQIQ YAQISVEMDVT |
| Post Translational Modifications | O-glycosylated. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. Ubiquitinated in the presence of TRIM2 and UBE2D1. |
| Function | Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks. |
| Protein Name | Neurofilament Light PolypeptideNf-L68 Kda Neurofilament ProteinNeurofilament Triplet L Protein |
| Database Links | Reactome: R-HSA-438066Reactome: R-HSA-442982Reactome: R-HSA-5673001Reactome: R-HSA-9609736Reactome: R-HSA-9617324Reactome: R-HSA-9620244 |
| Cellular Localisation | Cell ProjectionAxonCytoplasmCytoskeleton |
| Alternative Antibody Names | Anti-Neurofilament Light Polypeptide antibodyAnti-Nf-L antibodyAnti-68 Kda Neurofilament Protein antibodyAnti-Neurofilament Triplet L Protein antibodyAnti-NEFL antibodyAnti-NF68 antibodyAnti-NFL antibody |
Information sourced from Uniprot.org
